The study of biocompatibility of heart value transplants devitalized by anticalcinosis treatment



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Abstract

Earlier we have proposed method for anticalcinosis devitalization of heart valve transplants, which induced total cell death without elimination of cell debris from tissue matrix. It was shown in previous studies prevention of calcinosis in devitalized aorta transplants by the method of subcutaneous implantation in rats. In the present research we investigated biocompatibility of devitalized heart valve alio- and xenografts in the model of chronic implantation in descending aorta of dogs. It was revealed that viable and devitalized aorta grafts became acellular after 4-month implantation. Anticalcinosis divitalization of aorta allografts suppressed immune response, degeneration and calcinosis after their implantation in dogs. Xenografts after anticalcinosis treatment induced strong immune response resulted in fibrous reorganization of tissue matrix. According to the results obtained heart valve allografts devitalized by anticalcinosis treatment can be recommended for clinical trials.

About the authors

B C Akatov

P M Muratov

I S Fadeeva

A S Sachkov

D V Britikov

N I Fesenko

V V Solov'ev

A V Chekanov

V S Akatov

Institute of Theoretical and Experimental Biophysics of RAS, PushchinoPushchino State University, Pushchino

Institute of Theoretical and Experimental Biophysics of RAS, PushchinoPushchino State University, Pushchino

R M Muratov

Bakulev Center for Cardiovascular Surgery of RAMS, Moscow

Bakulev Center for Cardiovascular Surgery of RAMS, Moscow

I S Fadeeva

Institute of Theoretical and Experimental Biophysics of RAS, PushchinoPushchino State University, Pushchino

Institute of Theoretical and Experimental Biophysics of RAS, PushchinoPushchino State University, Pushchino

A S Sachkov

Bakulev Center for Cardiovascular Surgery of RAMS, Moscow

Bakulev Center for Cardiovascular Surgery of RAMS, Moscow

D V Britikov

Bakulev Center for Cardiovascular Surgery of RAMS, Moscow

Bakulev Center for Cardiovascular Surgery of RAMS, Moscow

N I Fesenko

Institute of Theoretical and Experimental Biophysics of RAS, PushchinoPushchino State University, Pushchino

Institute of Theoretical and Experimental Biophysics of RAS, PushchinoPushchino State University, Pushchino

V V Soloviev

Institute of Theoretical and Experimental Biophysics of RAS, PushchinoPushchino State University, Pushchino

Institute of Theoretical and Experimental Biophysics of RAS, PushchinoPushchino State University, Pushchino

A V Chekanov

Institute of Theoretical and Experimental Biophysics of RAS, PushchinoPushchino State University, Pushchino

Institute of Theoretical and Experimental Biophysics of RAS, PushchinoPushchino State University, Pushchino

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