Apoptosis-inducing activity of Bacillus pumilus ribonuclease and some egyptian medicinal plants extracts on human alveolar adenocarcinoma cells



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Induction of apoptosis is a primary mechanism of anticancer activity of several drugs using for cancer therapy. Apoptosis-inducing activity of Bacillus pumilus ribonuclease (binase) and stem bark aqueous extracts of eight medicinal plants from Egypt in human A549 alveolar adenocarcinoma cells was studied. It was shown that binase (300 μg/ml) significantly increases the portion of apoptic cells in population after 24 h. Extracts of Albizzia lebbeck and Bauhinia variegate demonstrated a clear dose-dependent apoptosis-inducing effect. Combined treatment of A549 cells with binase and stem bark aqueous extracts of Albizzia lebbeck, Bauhinia variegate, Kigelia africana enhances induction of apoptosis in comparison with binase and extracts alone. Results obtained allow to consider combination of binase and stem bark aqueous extracts of Albizzia lebbeck, Bauhinia variegate, Kigelia africana as a source for development of low toxic, natural drugs to induce apoptosis in tumor cells

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Во всех странах мира. По данным Международного агентства по исследованию рака в 2012 г. во всем мире было зарегистрировано 14 млн новых случаев заболеваний злокачественными опухолями и по прогнозам, за ближайшие 20 лет это число возрастет примерно на 70% [1]. На сегодняшний день сочетание хирургических методов лечения, лучевой и химиотерапии остается золотым стандартом лечения онкологических больных, что позволило повысить выживаемость пациентов за последние 3 , и выделения цитотоксических подофиллотоксинов [4]. Препараты на основе этих веществ сейчас успешно применяют для лечения детской лейкемии, лимфо-гранулломатоза, острого лейкоза, опухолей мягких тканей. Алкалоид паклитаксел (таксол), выделенный из тиса ягодного (Taxus baccata), - один из самых эффективных противоопухолевых препаратов в лечении рака молочной железы, яичников [5]. В настоящее время противоопухолевое действие показано для рибонуклеаз (РНКаз) - важнейших ферментов метаболизма РНК [6-10]. Особый интерес представляют собой РНКазы микробно
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作者简介

N. Karamova

Email: nskaramova@mail.ru
Kazan (Volga region) Federal University Assiut University Kazan, Russia Assiut, Egypt

P. Zelenikhin

Kazan (Volga region) Federal University Assiut University Kazan, Russia Assiut, Egypt

N. Miroshnik

Kazan (Volga region) Federal University Assiut University Kazan, Russia Assiut, Egypt

Abdul-Hafeez Essam

Kazan (Volga region) Federal University Assiut University Kazan, Russia Assiut, Egypt

Ya. Zakirova

Kazan (Volga region) Federal University Assiut University Kazan, Russia Assiut, Egypt

O. Ilinskaya

Kazan (Volga region) Federal University Assiut University Kazan, Russia Assiut, Egypt

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