Apoptosis-inducing activity of Bacillus pumilus ribonuclease and some egyptian medicinal plants extracts on human alveolar adenocarcinoma cells


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Abstract

Induction of apoptosis is a primary mechanism of anticancer activity of several drugs using for cancer therapy. Apoptosis-inducing activity of Bacillus pumilus ribonuclease (binase) and stem bark aqueous extracts of eight medicinal plants from Egypt in human A549 alveolar adenocarcinoma cells was studied. It was shown that binase (300 μg/ml) significantly increases the portion of apoptic cells in population after 24 h. Extracts of Albizzia lebbeck and Bauhinia variegate demonstrated a clear dose-dependent apoptosis-inducing effect. Combined treatment of A549 cells with binase and stem bark aqueous extracts of Albizzia lebbeck, Bauhinia variegate, Kigelia africana enhances induction of apoptosis in comparison with binase and extracts alone. Results obtained allow to consider combination of binase and stem bark aqueous extracts of Albizzia lebbeck, Bauhinia variegate, Kigelia africana as a source for development of low toxic, natural drugs to induce apoptosis in tumor cells

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About the authors

N. S Karamova

Email: nskaramova@mail.ru
Kazan (Volga region) Federal University Assiut University Kazan, Russia Assiut, Egypt

P. V Zelenikhin

Kazan (Volga region) Federal University Assiut University Kazan, Russia Assiut, Egypt

N. B Miroshnik

Kazan (Volga region) Federal University Assiut University Kazan, Russia Assiut, Egypt

Abdul-Hafeez Essam

Kazan (Volga region) Federal University Assiut University Kazan, Russia Assiut, Egypt

Ya. N Zakirova

Kazan (Volga region) Federal University Assiut University Kazan, Russia Assiut, Egypt

O. N Ilinskaya

Kazan (Volga region) Federal University Assiut University Kazan, Russia Assiut, Egypt

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