Nanodiamond composite scaffolds for human skin fibroblasts cultivation



Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Carbon materials are widely evaluated as scaffolds for cultured cells in “regenerative” bone surgery. In this research we investigated the interaction of human skin fibroblasts with diamond carbon composites scaffolds. Scaffolds with 10-100 nm pore diameter were obtained from the dispersed powders of diamond with particle size - from 2 nm to 5 microns It was shown that all scaffolds were not toxic for cultured cells. The highest number of cells adhered on the scaffolds with average pore size of 50-100 nm Scaffold with a pore size of 50 nm contribute to the fibroblasts parallel orientation of their surfaces

Full Text

Restricted Access

About the authors

Yu. A Nashchekina

Institute of Cytology of RAS

Email: yuliya.shved@gmail.com

B. A Margulis

Institute of Cytology of RAS

S. K Gordeev

Central Research Institute of Materials

M. I Blinova

Institute of Cytology of RAS

I. V Guzhova

Institute of Cytology of RAS

References

  1. Kim I. C. S., Yang T. K., Lee J. C. Radiological changes in the bone fusion site after posterior lumbar interbody fusion using carbon cages impacted with laminar bone chips: Eollow-up study over more than 4 years Spine 2005; 30(6): 655-60
  2. Bansal R. C. , Goyal M. Activated carbon adsorption Boca Raton: Taylor & Francis; 2005.
  3. Mooney E. , Drockery P., Greiser U. et al. Carbon nanotubes and mesenchimal stem cells: biocompatibility, proliferation and differentiation. Nanoletters 2008; 8(8): 2137-43.
  4. Yongbin Z., Syed Ali F. , Dervishi E. et al. Cytotoxicity effects of graphen and single-wall carbon nanotubes in neural phaechromacytomaderived PC12 cells. ACS NANO 2010; 4(6): 3181-6.
  5. Qiu Y. S., Shahgaldi B. F., Revell W.J. et al. Evaluation of Gateshead carbon fibre rod as an implant material for repair of osteochondral defects: A morphological and mechanical study in the rabbit knee Biomaterials 2002; 23: 3943-55
  6. Yu M. F., Files B. S., Arepalli S. et al. Tensile loading of ropes of single wall carbon nanotubes and their mechanical properties Phys Rev. Letters 2000; 84(24): 5552-5.
  7. Harrison B. S. , Atala A. Carbon nanotube applications for tissue engineering. Biomaterials 2007; 28(II): 344-53.
  8. Shvedova A.A. , Castranova V. , Kisin E. R. et al. Exposure to carbon nanotube material: assessment of nanotube cytotoxicity using human keratinocyte cells J Tox Env Health A 2003; 66(20): 1909-26.
  9. McKenzie J. L., Waid M. C., Shi R. et al. Decreased functions of astrocytes on carbon nanofiber materials J Biomaterials 2004; 25(7-8): 1309-17.
  10. Cui D. , Tian F. , Ozkan C. S. et al. Effect of single wall carbon nanotubes on human HEK293 cells. Toxicol. Lett. 2005; 155(1): 73-85
  11. Mattson M. P., Haddon R. C., Rao A. Molecular functionalization of carbon nanotubes and use as substrates for neuronal growth J Mol. Neurosci. 2000; 14(3): 175-82.
  12. Hu H. , Ni Y. , Montana V. et al. Chemically Functionalized Carbon Nanotubes as Substrates for Neuronal Growth Nano Lett 2004; 4(3): 507-11.
  13. Hu H. , Ni Y. , Mandal S. K. et al. Polyethyleneimine functionalized single-walled carbon nanotubes as a substrate for neuronal growth J Phys. Chem. B 2005; 109: 4285-9.
  14. Macdonald R. A. ; Laurenzi B. F. ; Viswanathan G. et al. Collagen-carbon nanotube composite materials as scaffolds in tissue engineering J Biomed Mater Res A 2005; 74: 489
  15. Wang J. H. C. , Grood E. S. , Florer J. et al. Alignment and proliferation of MC3T3-E1 osteoblasts in microgrooved silicone substrata subjected to cyclic stretching J Biomech 2000; 33: 729-35
  16. Ratner B. D. Biomaterials Science: An Introduction to Materials in Medicine 2nd ed London: Elsevier Academic Press; 2004
  17. Karageorgiou V. , Kaplan D. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 2005; 26: 5474-91.
  18. Гордеев С. К. Трехмерные углеродные наноматериалы Вопросы материаловедения 2008; 2: 163-74.
  19. Севостьянова А.А., Меленевский А. Т., Демин А.А. и др. Наноалмазы, импрегнированные в целлюлозную матрицу, для хроматографической очистки белков Сорбционные и хроматографические процессы 2011; 11(6):742-53.
  20. Vinante M. , Digregorio G. , Lunelli L. et al. Human plasma protein adsorption on carbon-based materials J Nanosci Nanotechnol 2009; 9(6): 3785-91

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2015 Eco-Vector


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: 

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies