Muscarinic regulation of calcium transient and synaptic transmission in frog neuromuscular junction



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In frog neuromuscular junction, muscarine, exogeneous acetylcholine and acetylcholinesterase inhibitor proserine reduced the intensity of calcium-sensitive dye fluorescence (calcium transient) at low frequency of nerve stimulation, suggesting that calcium ions entry into nerve ending was decreased. M2 muscarinic receptor blocker methoctramine prevented the action of muscarine. The amplitude of endplate currents was reduced in presence of muscarine at low frequency nerve stimulation, and atropine abolished this effect. Amplitudes of endplate currents evoked by high frequency stimulation were enhanced in presence of methoctramine, and synaptic depression was less pronounced, probably due to elevated calcium concentration in nerve ending. Thus, activation of presynaptic muscarinic receptors predominantly of M2 subtype reduces the intensity of quantal acetylcholine release in frog neuromuscular synapses that may be associated with decreased level of calcium ions in the nerve ending to provide the modulation of postsynaptic currents amplitude at high frequency firing.

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作者简介

D. Samigullin

Kazan Institute of Biochemistry and Biophysics of Russian Academy of Sciences; Kazan State Medical University; Kazan Federal (Volga region) University

E. Khaziev

Kazan Institute of Biochemistry and Biophysics of Russian Academy of Sciences; Kazan State Medical University; Kazan Federal (Volga region) University

I. Kovyazina

Kazan Institute of Biochemistry and Biophysics of Russian Academy of Sciences; Kazan State Medical University; Kazan Federal (Volga region) University

E. Bukharaeva

Kazan Institute of Biochemistry and Biophysics of Russian Academy of Sciences; Kazan State Medical University; Kazan Federal (Volga region) University

E. Nikolsky

Kazan Institute of Biochemistry and Biophysics of Russian Academy of Sciences; Kazan State Medical University; Kazan Federal (Volga region) University

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