Novel Surfaces for Improved Cultivation of Adherent and IMon-adherent Cells with Examples from Stem Cell Culture and 3D Tissue Engineering

Resumo

The presentation will give an overview of established and novel technologies to modify cultureware surfaces used in mammalian cell culture.
Cell harvesting using enzymatic digestion, such as trypsinization, results in degradation of cell surface proteins and extracellular matrix deposited by the cells. These proteins are important for the interactions between the cell and the environment. The Thermo Scientific Nunc™ UpCell™ Surface is designed to enable quick dissociation of cells from the culture surface upon a change in temperature. Depending on the degree of confluence of the culture, single cells or cell sheets can be harvested from the UpCell Surface. Examples from stem cell culture, as well as from 3D tissue engineering will be shown.
The unique feature of the new Thermo Scientific Nunc™ HydroCell™ surface is its ultra low cell binding capacity used for non-adherent cluster culture such as neural and embryonic stem cells.