Comparative investigation of polyhydroxyalkanoate scaffolds with various chemical compositions
- Autores: Nikolaeva ED1, Shishatskaya EI2, Mochalov KE3, Volova TG4, Sinsky AJ5
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Afiliações:
- Institute of Biophisycs, SB RAS, Krasnoyarsk
- Siberian Federal University, Krasnoyarsk
- Shemyakin and Ovchinnikov Institute of bioorganic chemistry RAS, Moscow
- Institute of Biophisycs, SB RAS, KrasnoyarskSiberian Federal University, Krasnoyarsk
- Massachusetts Institute of Technology, Cambridge, USA
- Edição: Volume 6, Nº 4 (2011)
- Páginas: 54-63
- Seção: Articles
- ##submission.dateSubmitted##: 11.01.2023
- ##submission.datePublished##: 15.12.2011
- URL: https://genescells.ru/2313-1829/article/view/121678
- DOI: https://doi.org/10.23868/gc121678
- ID: 121678
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Resumo
of membranes based on resorbable polyhydroxyalkanoates of
different compositions. Five PHA types have been studied: a
homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric
and 4-hydroxybutyric acids, 3-hydroxybutyric
and 3-hydroxyvaleric acids, 3-hydroxybutyric and 3-hydroxyhexanoic
acids. Scanning electron microscopy and atomicforce
microscopy were used to examine the microstructure
of membrane surfaces, showing that membranes based on
the copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate
had the roughest surface, while membranes based on the copolymer
of 3-hydroxybutyrate and 3-hydroxyvalerate had the
smoothest surface. The contact angle for water in air was
smaller and hydrophilic properties better in the copolymer
membranes than in the membranes based on the high-crystallinity
homopolymer of 3-hydroxybutyric acid. The culture of
mouse fibroblast cell line NIH 3Т3 was used to test PHAbased
membranes; results of fluorescent probes of DNA DAPI
and the MTT assay show that membranes based on studied
PHAs are not cytotoxic on direct contact with cells and are
highly biocompatible; their adhesive properties and ability to
maintain fibroblast proliferation are similar to those of polystyrene
and better than those of polylactic acid membranes.
Sobre autores
E Nikolaeva
Institute of Biophisycs, SB RAS, KrasnoyarskInstitute of Biophisycs, SB RAS, Krasnoyarsk
E Shishatskaya
Siberian Federal University, KrasnoyarskSiberian Federal University, Krasnoyarsk
K Mochalov
Shemyakin and Ovchinnikov Institute of bioorganic chemistry RAS, MoscowShemyakin and Ovchinnikov Institute of bioorganic chemistry RAS, Moscow
T Volova
Institute of Biophisycs, SB RAS, KrasnoyarskSiberian Federal University, KrasnoyarskInstitute of Biophisycs, SB RAS, KrasnoyarskSiberian Federal University, Krasnoyarsk
A Sinsky
Massachusetts Institute of Technology, Cambridge, USAMassachusetts Institute of Technology, Cambridge, USA
Bibliografia
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