Comparative investigation of polyhydroxyalkanoate scaffolds with various chemical compositions
- Authors: Nikolaeva ED1, Shishatskaya EI2, Mochalov KE3, Volova TG4, Sinsky AJ5
-
Affiliations:
- Institute of Biophisycs, SB RAS, Krasnoyarsk
- Siberian Federal University, Krasnoyarsk
- Shemyakin and Ovchinnikov Institute of bioorganic chemistry RAS, Moscow
- Institute of Biophisycs, SB RAS, KrasnoyarskSiberian Federal University, Krasnoyarsk
- Massachusetts Institute of Technology, Cambridge, USA
- Issue: Vol 6, No 4 (2011)
- Pages: 54-63
- Section: Articles
- Submitted: 11.01.2023
- Published: 15.12.2011
- URL: https://genescells.ru/2313-1829/article/view/121678
- DOI: https://doi.org/10.23868/gc121678
- ID: 121678
Cite item
Abstract
of membranes based on resorbable polyhydroxyalkanoates of
different compositions. Five PHA types have been studied: a
homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric
and 4-hydroxybutyric acids, 3-hydroxybutyric
and 3-hydroxyvaleric acids, 3-hydroxybutyric and 3-hydroxyhexanoic
acids. Scanning electron microscopy and atomicforce
microscopy were used to examine the microstructure
of membrane surfaces, showing that membranes based on
the copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate
had the roughest surface, while membranes based on the copolymer
of 3-hydroxybutyrate and 3-hydroxyvalerate had the
smoothest surface. The contact angle for water in air was
smaller and hydrophilic properties better in the copolymer
membranes than in the membranes based on the high-crystallinity
homopolymer of 3-hydroxybutyric acid. The culture of
mouse fibroblast cell line NIH 3Т3 was used to test PHAbased
membranes; results of fluorescent probes of DNA DAPI
and the MTT assay show that membranes based on studied
PHAs are not cytotoxic on direct contact with cells and are
highly biocompatible; their adhesive properties and ability to
maintain fibroblast proliferation are similar to those of polystyrene
and better than those of polylactic acid membranes.
About the authors
E D Nikolaeva
Institute of Biophisycs, SB RAS, KrasnoyarskInstitute of Biophisycs, SB RAS, Krasnoyarsk
E I Shishatskaya
Siberian Federal University, KrasnoyarskSiberian Federal University, Krasnoyarsk
K E Mochalov
Shemyakin and Ovchinnikov Institute of bioorganic chemistry RAS, MoscowShemyakin and Ovchinnikov Institute of bioorganic chemistry RAS, Moscow
T G Volova
Institute of Biophisycs, SB RAS, KrasnoyarskSiberian Federal University, KrasnoyarskInstitute of Biophisycs, SB RAS, KrasnoyarskSiberian Federal University, Krasnoyarsk
A J Sinsky
Massachusetts Institute of Technology, Cambridge, USAMassachusetts Institute of Technology, Cambridge, USA