Direct observation of «cholesterol - model of biological membrane» complex by NMR spectroscopy
- Autores: Galiullina, LF1, Blohin, DS1, Aganov, AV1, Klochkov VV1
-
Afiliações:
- Кazan (Volga region) Federal University, Kazan
- Edição: Volume 7, Nº 3 (2012)
- Páginas: 41-48
- Seção: Articles
- ##submission.dateSubmitted##: 11.01.2023
- ##submission.datePublished##: 15.09.2012
- URL: https://genescells.ru/2313-1829/article/view/121576
- DOI: https://doi.org/10.23868/gc121576
- ID: 121576
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Resumo
Interaction and aggregation of cholesterol and sodium
dodecyl sulfate molecules were studied in this paper.
Sodium dodecyl sulfate was taken as a model for biological
membranes. Cholesterol-sodium dodecyl sulfate complex was
described by modern methods of nuclear magnetic resonance
spectroscopy.
Nuclear magnetic resonance spectra were recorded on
«Avance-500» spectrometer (Bruker).
To assign 1Н signals of cholesterol, sodium dodecyl
sulfate and cholesterol+sodium dodecyl sulfate mixture
in nuclear magnetic resonance spectra literature data
was used, and 2D homo- end hetero-correlation nuclear
magnetic resonance spectra were recorded. To study the
formation of sodium dodecyl sulfate micelles and complex
of cholesterol-sodium dodecyl sulfate micelles selective
nuclear Overhauser effect spectroscopy experiments were
carried out.
The formation of sodium dodecyl sulfate micelles in dimethyl
sulfoxide solution was confirmed by nuclear Overhauser effect
spectroscopy data. The presence of a complex between sodium
dodecyl sulfate micelles and cholesterol molecules has been
proven by selective nuclear Overhauser effect spectroscopy
experiments. Nuclear Overhauser effect between OHgroup
of cholesterol and «tail» groups of sodium dodecyl
sulfate hydrophobic part was observed in the experiment.
This observation corresponds to close spatial arrangement
of these parts of different molecules and the presence of
a complex between cholesterol and sodium dodecyl sulfate
micelles.
On the basis of the nuclear magnetic resonance
experiments was established that molecules of sodium
dodecyl sulfate form micelles in dimethyl sulfoxide solution
at concentrations above the critical micelle concentration.
Cholesterol molecules form an intermolecular complex with
sodium dodecyl sulfate micelles by interaction of the OH group
of cholesterol and СН3-1 and СН2-2 «tail» aliphatic groups
of sodium dodecyl sulfate. This interaction is similar to the
behavior of cholesterol in phospholipid bilayer membranes in
which cholesterol enters its cyclic part in the hydrophobic
tails of phospholipid molecules oriented primarily across the
bilayers.
dodecyl sulfate molecules were studied in this paper.
Sodium dodecyl sulfate was taken as a model for biological
membranes. Cholesterol-sodium dodecyl sulfate complex was
described by modern methods of nuclear magnetic resonance
spectroscopy.
Nuclear magnetic resonance spectra were recorded on
«Avance-500» spectrometer (Bruker).
To assign 1Н signals of cholesterol, sodium dodecyl
sulfate and cholesterol+sodium dodecyl sulfate mixture
in nuclear magnetic resonance spectra literature data
was used, and 2D homo- end hetero-correlation nuclear
magnetic resonance spectra were recorded. To study the
formation of sodium dodecyl sulfate micelles and complex
of cholesterol-sodium dodecyl sulfate micelles selective
nuclear Overhauser effect spectroscopy experiments were
carried out.
The formation of sodium dodecyl sulfate micelles in dimethyl
sulfoxide solution was confirmed by nuclear Overhauser effect
spectroscopy data. The presence of a complex between sodium
dodecyl sulfate micelles and cholesterol molecules has been
proven by selective nuclear Overhauser effect spectroscopy
experiments. Nuclear Overhauser effect between OHgroup
of cholesterol and «tail» groups of sodium dodecyl
sulfate hydrophobic part was observed in the experiment.
This observation corresponds to close spatial arrangement
of these parts of different molecules and the presence of
a complex between cholesterol and sodium dodecyl sulfate
micelles.
On the basis of the nuclear magnetic resonance
experiments was established that molecules of sodium
dodecyl sulfate form micelles in dimethyl sulfoxide solution
at concentrations above the critical micelle concentration.
Cholesterol molecules form an intermolecular complex with
sodium dodecyl sulfate micelles by interaction of the OH group
of cholesterol and СН3-1 and СН2-2 «tail» aliphatic groups
of sodium dodecyl sulfate. This interaction is similar to the
behavior of cholesterol in phospholipid bilayer membranes in
which cholesterol enters its cyclic part in the hydrophobic
tails of phospholipid molecules oriented primarily across the
bilayers.
Sobre autores
L Galiullina,
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
D Blohin,
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
A Aganov,
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
V Klochkov
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
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