Direct observation of «cholesterol - model of biological membrane» complex by NMR spectroscopy
- Authors: Galiullina, LF1, Blohin, DS1, Aganov, AV1, Klochkov VV1
-
Affiliations:
- Кazan (Volga region) Federal University, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 41-48
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121576
- DOI: https://doi.org/10.23868/gc121576
- ID: 121576
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Abstract
Interaction and aggregation of cholesterol and sodium
dodecyl sulfate molecules were studied in this paper.
Sodium dodecyl sulfate was taken as a model for biological
membranes. Cholesterol-sodium dodecyl sulfate complex was
described by modern methods of nuclear magnetic resonance
spectroscopy.
Nuclear magnetic resonance spectra were recorded on
«Avance-500» spectrometer (Bruker).
To assign 1Н signals of cholesterol, sodium dodecyl
sulfate and cholesterol+sodium dodecyl sulfate mixture
in nuclear magnetic resonance spectra literature data
was used, and 2D homo- end hetero-correlation nuclear
magnetic resonance spectra were recorded. To study the
formation of sodium dodecyl sulfate micelles and complex
of cholesterol-sodium dodecyl sulfate micelles selective
nuclear Overhauser effect spectroscopy experiments were
carried out.
The formation of sodium dodecyl sulfate micelles in dimethyl
sulfoxide solution was confirmed by nuclear Overhauser effect
spectroscopy data. The presence of a complex between sodium
dodecyl sulfate micelles and cholesterol molecules has been
proven by selective nuclear Overhauser effect spectroscopy
experiments. Nuclear Overhauser effect between OHgroup
of cholesterol and «tail» groups of sodium dodecyl
sulfate hydrophobic part was observed in the experiment.
This observation corresponds to close spatial arrangement
of these parts of different molecules and the presence of
a complex between cholesterol and sodium dodecyl sulfate
micelles.
On the basis of the nuclear magnetic resonance
experiments was established that molecules of sodium
dodecyl sulfate form micelles in dimethyl sulfoxide solution
at concentrations above the critical micelle concentration.
Cholesterol molecules form an intermolecular complex with
sodium dodecyl sulfate micelles by interaction of the OH group
of cholesterol and СН3-1 and СН2-2 «tail» aliphatic groups
of sodium dodecyl sulfate. This interaction is similar to the
behavior of cholesterol in phospholipid bilayer membranes in
which cholesterol enters its cyclic part in the hydrophobic
tails of phospholipid molecules oriented primarily across the
bilayers.
dodecyl sulfate molecules were studied in this paper.
Sodium dodecyl sulfate was taken as a model for biological
membranes. Cholesterol-sodium dodecyl sulfate complex was
described by modern methods of nuclear magnetic resonance
spectroscopy.
Nuclear magnetic resonance spectra were recorded on
«Avance-500» spectrometer (Bruker).
To assign 1Н signals of cholesterol, sodium dodecyl
sulfate and cholesterol+sodium dodecyl sulfate mixture
in nuclear magnetic resonance spectra literature data
was used, and 2D homo- end hetero-correlation nuclear
magnetic resonance spectra were recorded. To study the
formation of sodium dodecyl sulfate micelles and complex
of cholesterol-sodium dodecyl sulfate micelles selective
nuclear Overhauser effect spectroscopy experiments were
carried out.
The formation of sodium dodecyl sulfate micelles in dimethyl
sulfoxide solution was confirmed by nuclear Overhauser effect
spectroscopy data. The presence of a complex between sodium
dodecyl sulfate micelles and cholesterol molecules has been
proven by selective nuclear Overhauser effect spectroscopy
experiments. Nuclear Overhauser effect between OHgroup
of cholesterol and «tail» groups of sodium dodecyl
sulfate hydrophobic part was observed in the experiment.
This observation corresponds to close spatial arrangement
of these parts of different molecules and the presence of
a complex between cholesterol and sodium dodecyl sulfate
micelles.
On the basis of the nuclear magnetic resonance
experiments was established that molecules of sodium
dodecyl sulfate form micelles in dimethyl sulfoxide solution
at concentrations above the critical micelle concentration.
Cholesterol molecules form an intermolecular complex with
sodium dodecyl sulfate micelles by interaction of the OH group
of cholesterol and СН3-1 and СН2-2 «tail» aliphatic groups
of sodium dodecyl sulfate. This interaction is similar to the
behavior of cholesterol in phospholipid bilayer membranes in
which cholesterol enters its cyclic part in the hydrophobic
tails of phospholipid molecules oriented primarily across the
bilayers.
About the authors
L F Galiullina,
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
D S Blohin,
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
A V Aganov,
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
V V Klochkov
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
References
- Yeagle P.L. Cholesterol and the cell membrane. Biochim. Biophys. Acta. 1985; 822: 267-87.
- Borroni B., Pettenati C., Bordonali T. et al. Serum cholesterol levels modulate long-term efficacy of cholinesterase inhibitors in Alzherimer disease. Neuroscience Lett. 2003; 343: 213-5.
- Mironov V.S., Galyametdinov Y.G., Ceulemans A. et al. J. Chem. Phys. 2000; 113: 10293-303.
- Wang G., Keifer P., Peterkofsky A. Solution structure of the N-terminal amphitropic domain of Escherichia coli glucose-specific enzyme IIA in membrane-mimetic micelles. Protein Science 2003; 12: 1087-96.
- Blokhin D.S., Efimov S.V., Klochkov A.V. et al. Spatial structure of the decapeptide Val-Ile-Lys-Lys-Ser-Thr-Ala-Leu-Leu-Gly in water and in a complex with sodium dodecyl sulfate micelles. Appl. Magn. Reson. 2011; 41(2): 267-82.
- Henry G.D., Sykes B.D. Methods to study membrane protein structure in solution. Methods in Enzymology 1994; 239: 515-35.
- Stonehouse J., Adell P., Keeler J. et al. Ultrahigh-quality NOE spectra. J. Am. Chem. Soc. 1994; 116: 6037-8.
- Stott K., Stonehouse J., Keeler J. et al. Excitation sculpting in high-resolution nuclear magnetic resonance spectroscopy: application to selective NOE experiments. J. Am. Chem. Soc. 1995; 117: 4199-200.
- Castagne D., Dive G., Evrard B. et al. Spectroscopic studies and molecular modeling for understanding the interactions between cholesterol and cyclodextrins. J. Pharm. Pharmaceut. Sci. 2010; 13(2): 362-77
- Singh H.N., Singh S., Tewari K.S. Surface active anions in polar solvents: Conductometric studies on solutions of sodium decyl and dodecyl sulfate in water, N,N-dimethylformamide, and dimethyl sulfoxide. Journal of the American oil chemists society. 1995; 52: 436-8.
- Ernst R.R., Bodenhausen B., Wokaun A. Principles of nuclear magnetic resonance in one and two dimensions. Oxford: Oxford University Press; 1987.
- Gadiev T.A., Khairutdinov B.I., Antipin I.S. et al. Analysis of the spatial structure of calixarenes in solutions by 2-D NMR (NOESY) spectroscopy. Appl. Magn. Reson. 2006; 30(2): 65-73.
- Gordon S.R., Hitchens T.K. Fundamentals of Protein NMR spectroscopy. Dordrecht: Springer; 2006.
- Elles Ch.G., Levinger N.E. Reverse micelles solubilizing DMSO and DMSO-water mixtures. Chemical Physics Letters 2000; 317: 624-30.
- Robinson A.J.; Richards W.G., Thomas P.J. et al. Behavior of сholesterol and its effect on head group and chain conformations in lipid bilayers: a molecular dynamics study. Biophysical Journal 1995; 68: 164-70.
- Tiburu E.K., Dave P.C., Lorigan G.A. Solid-state 2H NMR studies of the effects of cholesterol on the acyl chain dynamics of magnetically aligned phospholipid bilayers. Magn. Reson. Chem. 2004; 42: 132-8.
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