Tryptophanyl-tRNA synthase gene expression as genetic marker of the athletes’ overtraining

In connection with a necessity the development of effective biomarkers of sportsman physical and overtraining (distress) states, the study of a role of triptophanyl-tRNA synthase, TRSase, and corresponding gene in process is of special interest. Preliminary estimate of specificity and level of mRNA expression of TRSase gene is carried out using method 1: scanning of gels with an image of separation of polymerase chain reaction PCR products to detect quantitatively the content of the mRNA and/or cDNA copies compare, in parallel, to DNA quantity markers to compose a calibrating curve. Final estimation of TRSase gene expression is carries out by real time PCR (method 2) using calibrating curve (technique 1) and relative quantitative estimation of a number of specific mRNA copies with reference gene involved (technique 2). The level of TRSase gene expression represents a criterium of organism response, which is adequate to a stress strength (overtraining) It is proposed to detect the overtraining state at sportsmen using registrating an increased TRSase gene expression. In our pilot project, the distress overtraining state is determined as increased level of specific TRSase gene expression in 1,45 fold higher at the samples under the study compare to control samples. The TRSase mRNA expression before training is increased by 1 2-1 6 fold compare to one after training

sportsman overtraining, eustress, distress, triptophanyl-tRNA synthase gene, an increase in TRSase gene expression as overtraining biomarker