Binase induces pro-inflammatory immune response of macrophages



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Resumo

Biopharmaceutical agents, especially proteins, may possess a broad spectrum of immunological effects. Cytotoxic ribonucleases, such as binase - RNase of Bacillus pumilus, have a significant potential as anticancer therapeutical agents. However without comprehensive analysis of immune system reactions on the proteins, the practical utility of them is complicated. Using the flow cytometry we estimated qualitative and quantitative changes in IL-6, IL-10, МСР-1 and TNF-acytokines synthesis by peripheral macrophages of mice under the influence of binase and RNAse A. We established that binase in concentration 100 and 300 jg/ml, in contrast to RNase A, is able to activate pro-inflammatory response of macrophages, inducing increase of IL-6, МСР-1 andTNF-a cytokines level in cultural media. Treatment with RNases does not enhance the antiinflammatory cytokime IL-10 synthesis. Obtained data lead as to the assumption, that binase capability to activate pro-inflammatory response of macrophages will intensify its antitumor effects in vivo.

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Sobre autores

A. Lozhkin

Kazan (Volga Region) Federal University

P. Zelenikhin

Kazan (Volga Region) Federal University

A. Makeeva

Kazan (Volga Region) Federal University

E. Sokolova

Kazan (Volga Region) Federal University

O. Ilinskaya

Kazan (Volga Region) Federal University

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