Influence of some metabolites from plants of the genus  Petasítes  sp. on cancer cells motility in vitro

S. Yu. Filippova; Филиппова С. Ю.; T. V. Shamova; Шамова Т. В.; S. V. Timofeeva; Тимофеева С. В.; A. O. Sitkovskaya; Ситковская А. О.; I. V. Mezhevova; Межевова И. В.; N. V. Gnennaya; Гненная Н. В.; I. A. Novikova; Новикова И. А.; Ya. S. Enin; Енин Я. С.; O. N. Burov; Буров О. Н.; E. Yu. Zlatnik; Златник Е. Ю.; S. S. Mezentsev; Мезенцев С. С.; E. N. Chernikova; Черникова Е. Н.; O. V. Pandova; Пандова О. В.; V. V. Pozdnyakova; Позднякова В. В.; M. O. Ezhova; Ежова М. О.; S. M. Bakulina; Бакулина С. М.; O. V. Khokhlova; Хохлова О. В.

doi:10.23868/202209009

Influence of some metabolites from plants of the genus Petasítes sp. on cancer cells motility in vitro

Authors: Filippova S.Y.¹, Shamova T.V.¹, Timofeeva S.V.¹, Sitkovskaya A.O.¹, Mezhevova I.V.¹, Gnennaya N.V.¹, Novikova I.A.¹, Enin Y.S.¹, Burov O.N.², Zlatnik E.Y.¹, Mezentsev S.S.¹, Chernikova E.N.¹, Pandova O.V.¹, Pozdnyakova V.V.¹, Ezhova M.O.¹, Bakulina S.M.¹, Khokhlova O.V.¹
Affiliations:
1. National medical research center for oncology
2. Southern federal university
Issue: Vol 17, No 2 (2022)
Pages: 60-63
Section: Original Study Articles
URL: https://genescells.ru/2313-1829/article/view/133721
DOI: https://doi.org/10.23868/202209009
ID: 133721

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Abstract

Secondary plant metabolites are a promising source of anticancer drugs. Of particular interest are substances that can reduce the migration activity of cancer cells as potential anti-metastatic drugs. In present work we study the influence of 2,4-dihydroxy-2,5-dimethylfuran-3(2H)-one, 5-(hydroxymethyl)furan-2-carbaldehyde and corinan obtained from plants of the genus Petasítes sp. on scratch healing rate of permanent cancer lines PC3, A431, CaCo2, HeLa, and T98G. Cells were grown in DMEM medium supplemented with 10% FBS. To perform a scratch test, cells were planted in an amount of 1,5×10⁵ per well of a 24-well plate. After cell adhesion, a vertical scratch was applied to the cell monolayer with a plastic tip, after which the medium containing the test substances at a concentration of 40 µM was added. Then, within 48 hours, photographing and determining the scratch area were carried out. The degree of scratch overgrowth was determined as the ratio of the difference between the scratch areas after 48 hours of cultivation and the scratch area at the initial moment, expressed as a percentage. As a result of the experiment, it was shown that 2,4-dihydroxy-2,5-dimethylfuran-3(2H)-one downregulates scratch healing rate in cultures A431, HeLa, T98G. The high sensitivity of A431 to corinan was also shown.

Keywords

plant secondary metabolites, tumor cell cultures, anti-migratory activity

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