Bioresorption and biodegradation of the 3D-printed gene-activated bone substitutes based on octacalcium phosphate
- Authors: Presnyakov E.V1, Bozo I.Y2,3, Smirnov I.V4, Komlev V.S4,5, Popov V.K5, Mironov A.V5, Deev R.V6,7
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Affiliations:
- I.P. Pavlov Ryazan State Medical University
- A.I. Burnazyana Federal Medical Biophysical Center, FMBA of Russia
- Histograft, LLC
- A.A. Baikov Institute of Metallurgy and Materials Science, RAS
- Federal Research Center “Crystallography and photonics”, RAS
- Institute of Human Stem Cells, PJSC
- I.I. Mechnikov North-Western State Medical University
- Issue: Vol 15, No 1 (2020)
- Pages: 66-70
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/122059
- DOI: https://doi.org/10.23868/202003009
- ID: 122059
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Abstract
Gene-activated materials are getting translated to the clinical practice that is the result of increased research activity in this area. There is significant success in development of gene-activated materials for bone grafting procedures. In our study, we made the gene-activated bone substitute based on octacalcium phosphate and plasmid DNA with VEGFA gene using three-dimensional printing technologies (discs, size of 10x2 mm). During the subcutaneous test in rats, the dynamics of bioresorption of materials was evaluated in comparison with the control that was presented by the scaffold without gene constructs. 60-90 days after surgery, volumes of the implants twicely decreased while the diameter declined by 20% at 180 days, and there were no significant differences between the groups related to these parameters. According to a histological examination, no signs of pronounced inflammation were found in the operation zone, the materials were surrounded by a connective tissue capsule. Further studies are needed to evaluate the effect of gene-activated materials produced with the developed technology on reparative osteogenesis.
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About the authors
E. V Presnyakov
I.P. Pavlov Ryazan State Medical University
I. Y Bozo
A.I. Burnazyana Federal Medical Biophysical Center, FMBA of Russia; Histograft, LLC
I. V Smirnov
A.A. Baikov Institute of Metallurgy and Materials Science, RAS
V. S Komlev
A.A. Baikov Institute of Metallurgy and Materials Science, RAS; Federal Research Center “Crystallography and photonics”, RAS
V. K Popov
Federal Research Center “Crystallography and photonics”, RAS
A. V Mironov
Federal Research Center “Crystallography and photonics”, RAS
R. V Deev
Institute of Human Stem Cells, PJSC; I.I. Mechnikov North-Western State Medical University
References
- Goodson A.M., Kittur M.A., Evans P.L. et. al. Patient-specific, printed titanium implants for reconstruction of mandibular continuity defects: A systematic review of the evidence. J Craniomaxillofac Surg. 2019; 47(6): 968-76.
- Ren W.H., Gao L., Li S.M. et al.Virtual planning and 3D printing modeling for mandibular reconstruction with fibula free flap. Zhonghua Yi Xue Za Zhi. 2018; 98(33): 2666-2670.
- Scarano A, Stoppaccioli M, Casolino T. Zirconia crowns cemented on titanium bars using CAD/CAM: a five-year follow-up prospective clinical study of 9 patients. BMC Oral Health. 2019; 19(1): 286.
- Guillaume B. Filling bone defects with p-TCP in maxillofacial surgery: A review. Morphologie. 2017; 101(334): 113-9.
- Komlev V.S., Barinov S.M., Bozo 1.1. et al. Bioceramics composed of octacalcium phosphate demonstrate enhanced biological behaviour. ACS Appl. Mater. Interfaces 2014; 6(19): 16610-20.
- Komlev V.S., Popov V.K., Mironov A.V. et al. 3D printing of octacalcium phosphate bone substitutes. Front Bioeng Biotechnol. 2015; 3: 81.
- Deev R.V., Bozo I.Y., Mzhavanadze N.D. et al. pCMV-vegf165 intramuscular gene transfer is an effective method of treatment for patients with chronic lower limb ischemia. J Cardiovasc Pharmacol Ther. 2015; 20(5): 473-82.
- Деев Р.В., Бозо И.Я., Мжаванадзе Н.Д. и др. Эффективность применения гена VEGF165 в комплексном лечении пациентов с хронической ишемией нижних конечностей 2а-3 стадии. Ангиология и сосудистая хирургия 2014; 20(2): 38-48
- Бонарцев А.П., Мураев А.А., Деев Р.В., Волков А.В. Материал-ассоциированная костная резорбция. Современные технологии в медицине 2018; 10(4): 26-33
- Бозо И.Я., Деев Р.В., Дробышев А.Ю. и др. Эффективность ген-активированного остеопластического материала на основе окта-кальциевого фосфата и плазмидной днк с геном VEGF в восполнении «критических» костных дефектов. Вестник травматологии и ортопедии им. Н.Н. Приорова 2015; 1: 35-42
- Animal Welfare Act; 2013. https://www.nal.usda.gov/awic/ animal-welfare-act
- Деев Р.В., Дробышев А.Ю., Бозо И.Я. Ординарные и активированные остеопластические материалы. Вестник травматологии и ортопедии им. Н.Н. Приорова 2015; 1: 51-69.
- ГОСТ ISO 10993-6-2011 Изделия медицинские. Оценка биологического действия медицинских изделий. Часть 6. Исследования местного действия после имплантации
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