Investigation of telomere length and umbilical cord blood hematopoietic stem cells colony-formingpotential in cryoconservation
- Authors: Karpova, NS1, Abdulkadyrov, KM1, Selivanov, EA1, Балашова ВА1
-
Affiliations:
- Russian Research Institute of Hematology and Transfusiology of the Russian Federal Bio-Medical Agency,Saint-Petersburg
- Issue: Vol 6, No 4 (2011)
- Pages: 42-47
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121671
- DOI: https://doi.org/10.23868/gc121671
- ID: 121671
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Abstract
human hematopoietic cord blood stem cells at two different
methods of cryopreservation. The proliferative potential was
determined by measurement of telomere length and colonyforming
cell assay. The telomere length was estimated by
technique that combined flow cytometry and fluorescence
in situ hybridization on Beckman Coulter Cytomics FS-500.
We used the Telomere PNA Kit/FITC for Flow Cytometry for
measuring telomeric sequences as well as MethoCult® GF
H4435 in colony-forming cell assays. The programmed Planer
Cryo freezer and direct transfer into liquid nitrogen vapour
were used for cryopreservation of samples. The correlation
between telomere length and capacity of cells to forming colony
after thawing were established. The programmed freezing
showed advantage over freezing by direct transfer cells
into liquid nitrogen vapour by better keeping of proliferative
capacity hematopoietic cells.
About the authors
N S Karpova,
Russian Research Institute of Hematology and Transfusiology of the Russian Federal Bio-Medical Agency,Saint-PetersburgRussian Research Institute of Hematology and Transfusiology of the Russian Federal Bio-Medical Agency,Saint-Petersburg
K M Abdulkadyrov,
Russian Research Institute of Hematology and Transfusiology of the Russian Federal Bio-Medical Agency,Saint-PetersburgRussian Research Institute of Hematology and Transfusiology of the Russian Federal Bio-Medical Agency,Saint-Petersburg
E A Selivanov,
Russian Research Institute of Hematology and Transfusiology of the Russian Federal Bio-Medical Agency,Saint-PetersburgRussian Research Institute of Hematology and Transfusiology of the Russian Federal Bio-Medical Agency,Saint-Petersburg
В А Балашова
Russian Research Institute of Hematology and Transfusiology of the Russian Federal Bio-Medical Agency,Saint-PetersburgRussian Research Institute of Hematology and Transfusiology of the Russian Federal Bio-Medical Agency,Saint-Petersburg
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