Influence of recombinant histone H1.3 on the efficiency of lentiviral transduction of human cells in vitro
- Authors: Solovyeva VV1, Isaev AA2, Genkin DD3, Rizvanov AA1
-
Affiliations:
- Kazan (Volga region) federal university, Kazan
- Human Stem Cells Institute, Moscow
- OJSC «Pharmsynthez», Saint-Petersburg
- Issue: Vol 7, No 3 (2012)
- Pages: 151-154
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121657
- DOI: https://doi.org/10.23868/gc121657
- ID: 121657
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Abstract
Lentiviral vectors are widely used in genetic modification
of human and animal cells (lentiviral transduction) to enhance
their therapeutic potential by expression of recombinant
protective and trophic factors. Genetic modification of cells in
vitro or ex vivo achieves the specificity of viral transduction,
as modified are just cells that have been manipulated in
the laboratory. In addition, the introduction of genetically
modified cells, but not pure virus, helps to avoid introduction
of viral particles into the body of the recipient. This approach
allows us to control the expression of therapeutic genes, the
immunogenicity of viral vectors and viral transduction. To
date, different approaches are used to improve the lentiviral
transduction (polycations, protamine sulfate, etc.), but these
methods suffer from limited efficacy or high toxicity. For the
first time we demonstrated that the recombinant histone
N1.3 increases the efficiency of lentiviral transduction by
more than 2 times and has no toxic effect on target cells in
a wide range of concentrations studied.
of human and animal cells (lentiviral transduction) to enhance
their therapeutic potential by expression of recombinant
protective and trophic factors. Genetic modification of cells in
vitro or ex vivo achieves the specificity of viral transduction,
as modified are just cells that have been manipulated in
the laboratory. In addition, the introduction of genetically
modified cells, but not pure virus, helps to avoid introduction
of viral particles into the body of the recipient. This approach
allows us to control the expression of therapeutic genes, the
immunogenicity of viral vectors and viral transduction. To
date, different approaches are used to improve the lentiviral
transduction (polycations, protamine sulfate, etc.), but these
methods suffer from limited efficacy or high toxicity. For the
first time we demonstrated that the recombinant histone
N1.3 increases the efficiency of lentiviral transduction by
more than 2 times and has no toxic effect on target cells in
a wide range of concentrations studied.
Keywords
About the authors
V V Solovyeva
Kazan (Volga region) federal university, KazanKazan (Volga region) federal university, Kazan
A A Isaev
Human Stem Cells Institute, MoscowHuman Stem Cells Institute, Moscow
D D Genkin
OJSC «Pharmsynthez», Saint-PetersburgOJSC «Pharmsynthez», Saint-Petersburg
A A Rizvanov
Kazan (Volga region) federal university, KazanKazan (Volga region) federal university, Kazan
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