Current state of EDTA-associated pseudothrombocytopenia, importance in the pathogenesis of polymorphism in the gene of platelet receptor for fibrinogen



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Abstract

EDTA-associated pseudothrombocytopenia a laboratory phenomen associated with the widespread introduction into laboratory practice of hematology analyzers and standardized methods for blood samples preserving. High frequency pseudothrombocytopenia among healthy individuals (20%) and among patients with various pathological conditions (50%), and lack of control of the results, can cause a large amount of laboratory and diagnostic errors. Currently there is no reliable data on the clinical value pseudothrombocytopenia. Identify EDTA-pseudothrombocytopenia based on a comparison of the data automated hematological analysis with the results of microscopic examination platelets count or automated studies using alternative anticoagulants. Detection of somatic mutations in the platelet fibrinogen receptor gene (integrin gene polymorphism ITGB3-b: 1565 T>C) in a limited study - 63.2% (12 patients surveyed from 1 9) may serve as the beginning of a new direction in understanding the etiopathogenesis and clinical significance of pseudothrombocytopenia.

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About the authors

A. S Polyakov

S.M. Kirov Military Medical Academy

Saint Petersburg, Russia

E. V Goncharova

S.M. Kirov Military Medical Academy

Saint Petersburg, Russia

S. G Bologov

S.M. Kirov Military Medical Academy

Saint Petersburg, Russia

D. K Zhogolev

S.M. Kirov Military Medical Academy

Saint Petersburg, Russia

S. V Bondarchuk

S.M. Kirov Military Medical Academy

Saint Petersburg, Russia

Ya. A Noskov

S.M. Kirov Military Medical Academy

Saint Petersburg, Russia

O. R Petrova

S.M. Kirov Military Medical Academy

Saint Petersburg, Russia

S. V Voronin

S.M. Kirov Military Medical Academy

Saint Petersburg, Russia

S. N Kolubaeva

S.M. Kirov Military Medical Academy

Saint Petersburg, Russia

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