Reversible epithelio-mesenchymal transformations of cells in embryogenesis and postnatal tissue regeneration

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Abstract

The modern findings on gene expression interchanging that characterize manifestation of epithelial and mesodermal (mesenchymal) cells within early embryogenesis are presented. Based on our data obtained while using immunocytochemisrty method (CD43, CD45, CD14, CD44, CD54, CD56, CD90, CD105 antibodies) the authors consider the part of mesenchymal stem cells to be epithelial ones. It is suggested that interchanging of epithelial and mesenchymal phenotype features are general biologic natural phenomenon in ontogenesis.

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About the authors

V. S. Repin

A.Ya. Friedenshtein Research Institute of cellular technologies and regenerative medicine

Author for correspondence.
Email: redaktor@celltranspl.ru
Russian Federation, Moscow

I. N. Saburina

A.Ya. Friedenshtein Research Institute of cellular technologies and regenerative medicine

Email: redaktor@celltranspl.ru
Russian Federation, Moscow

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Supplementary files

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2. Fig.1. Morphology of human bone marrow cell culture (lens S20 ): A – epithelioid colony; B – fibroblast - like Bacolod

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3. Fig. 2. Immunohistochemical analysis of epithelioid cell colonies from bone marrow:A - expression of nestin (lens x20); B - expression of E-cadherin (lens x20); C - co-expression of nestin and vimentin (lens x10); D- expression of a-actin MMC (lens x40)Pre-painting of cores - Hoechst

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6. Fig. 3. Immunohistochemical analysis of fibroblast–like cell colonies from bone marrow: A - expression of E-cadherin (lens x20); B - expression of nestin (lens x20); C - expression of α-actin MMC (lens x40) Pre–coloring of nuclei - Hoechst

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7. Fig. 4. Expression in MSCS culture isolated from bone marrow: von Willebrand A-factor (VWF); B – CD105 (х40 lens) Pre–painting of cores - Hoechst

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8. Figure 5. Immunohistochemical analysis of postnatal progenitor cells from human adipose tissue:A - expression of E-cadherin (lens x30); B — co—expression of nestin and vimentin (lens x10); C - expression of a-actin MMC (lens x40)Pre—painting of cores - Hoechst

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9. Fig.5_2

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