Dynamics of inflammatory infiltrate in mice liver under conditions of experimental fecal peritonitis
- Authors: Safronova G.M.1, Indeikin F.A.1, Andreev Y.A.1, Emelin A.M.1, Presnyakov E.V.2, Deev R.V.1,2
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Affiliations:
- I.I. Mechnikov North-Western State Medical University
- Histograft LLC
- Issue: Vol 17, No 2 (2022)
- Pages: 64-66
- Section: Original Study Articles
- URL: https://genescells.ru/2313-1829/article/view/133724
- DOI: https://doi.org/10.23868/202209011
- ID: 133724
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Abstract
The relationship between the liver and the peritoneum is an important element in a body homeostasis maintaining. Morphofunctional changes occurring in the liver in response to fecal peritonitis allow a better understanding of this relationship. Despite the existing researches, some aspects of the pathological changes occurring in the liver in fecal peritonitis require a special study.
Purpose: to describe the composition and dynamics of the lymphocytic-leukocytic infiltrate in the liver in conditions of experimental fecal peritonitis.
The study was conducted on Balb/c (n=5) mice with artificially induced fecal peritonitis. One animal was euthanized on 1, 3, 5, 7 and 14 day of the experiment. Immunophenotyping of inflammatory infiltrate cells was performed on liver tissue samples using antibodies to CD3, CD45, CD163. Morphometric analysis was carried out with the calculation of the number of cells per unit area.
It was shown that the highest intensity of infiltration by CD45+-cells is observed on the 3rd day of the experiment with a subsequent decrease; infiltration by CD3+-cells increases by the 5th day of the experiment without further dynamics; infiltration of CD163+-cells — by day 7 without further changes.
Conclusions: the severity of the liver parenchyma infiltration by immune cells in dynamics reflects the organ reaction to experimental fecal peritonitis.
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About the authors
G. M. Safronova
I.I. Mechnikov North-Western State Medical University
Author for correspondence.
Email: f.indeickin@yandex.ru
Russian Federation, St. Petersburg
F. A. Indeikin
I.I. Mechnikov North-Western State Medical University
Email: f.indeickin@yandex.ru
Russian Federation, St. Petersburg
Yu. A. Andreev
I.I. Mechnikov North-Western State Medical University
Email: f.indeickin@yandex.ru
Russian Federation, St. Petersburg
A. M. Emelin
I.I. Mechnikov North-Western State Medical University
Email: f.indeickin@yandex.ru
Russian Federation, St. Petersburg
E. V. Presnyakov
Histograft LLC
Email: f.indeickin@yandex.ru
Russian Federation, Moscow
R. V. Deev
I.I. Mechnikov North-Western State Medical University; Histograft LLC
Email: f.indeickin@yandex.ru
Russian Federation, St. Petersburg; Moscow
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