Stimulating effect of FS-1 on functional activity of lymphopoiesis and myelopoiesis progenitor cells in vitro

Abstract

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Cancer is the second leading cause of death globally, and is responsible for an estimated 9,6 million deaths in 2018. Chemotherapy is a cancer treatment that uses drugs to kill rapidly growing and dividing cells, like cancer cells. Unlike radiation or surgery, which target specific areas, chemo works systemically. This affects some fast-growing healthy cells, like those of the skin, hair, intestines, and bone marrow. Bone marrow is the spongy tissue inside the bones, which makes new blood cells. Chemotherapy affects this process, so there are side effects, such as too few blood cells. Low levels of blood cells during treatment can cause problems: more susceptible to infections; body tissues do not receive enough oxygen (hypoxia); anemia and increased blood coagulation. Prevention and treatment of such side effects is called palliative or supportive therapy and constitutes an important part in cancer treatment. In this article we present study results concerning new medicine FS-1 effect on bone marrow mesenchymal stem cells. FS-1 synthesized at Scientific Centre for Anti-infectious Drugs is ionic nanostructured complex formed by proteins and/or polypeptides, hydrocarbons, salts of alkali and alkaline earth metals with intercalated iodine. Patented in 2014 (RK No20129000). In our work we used mesenchymal stem cells from mouse bone marrow (STEMCELLS Technologies, USA). Detect production of stem-cells factor (SCF), IL-7, IL-8 macrophage colony-stimulating factor (M-CSF), chemotactic stroma-derived factor-1 (SDF-1 ), macrophage anti-inflammatory protein (MIP)-1a and MIP-1p quantitative analysis was made using kits from R&D Systems (USA) in accordance with manufacturers' protocols. In the process of study of FS-1 effect on production of cytokines and chemokines in mouse bone marrow mesenchymal stem cells culture we found out that FS-1 has actual stimulating effect on production of SCF (Р = 0,015), IL-7 (Р = 0,017), M-CSF (Р = 0,009) and SDF-1 (P = 0,009). Thus, new medicine FS-1 can influence animal bone marrow by stimulating mouse bone marrow mesenchymal stem cells to produce certain cytokines regulating myelopoiesis and lymphopoiesis, as well as certain chemokines taking part in induction of stem cells and mature cells migration from bone marrow to blood. Obtained results can be used in development of drugs for bone marrow hematopoietic function recovery during chemotherapy of tumors.