Application of electroanalytical techniques for assessment of human cells

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Abstract

Rapid development of cell technologies stipulates the need
for informative and express methods for the analysis of viability
and physiological activity of human cells. We studied analytical
possibilities of novel tools for comprehensive characterization
of bioelectrochemical properties of living cells, e.g. surface
charge of cellular membrane and redox activity of metabolites.
Using Malvern Zetasizer dynamic light scattering analyzer we
proposed an approach to assessment of zeta potential of human
cells and detection of phosphatidylserine on their surface as
an early apoptotic marker. On the basis of modified electrodes
we designed sensors exhibiting high sensitivity towards
electroactive cellular metabolites including antioxidants
and macroergic molecules. The sensors were applied for
assessment of metabolic activity/energetic status of human
cells (blood cells and cell cultures). Electrochemical signal of
adenine nucleotides of cells on sensor surface correlated with
intracellular level of ATP according to luciferase assay and was
found to be more sensitive to alteration in cell viability than
conventional MTS test. On the basis of disposable screenprinted
electrodes we fabricated a prototype of portable
analyzer for rapid analysis of cell health (e.g. for donor cells)
in less than 5 ƒl volume of cell sample. Proposed tools and
methods are of interest in cell transplantology, basic research
and cell-based medical diagnostics.

About the authors

D V Saifullina

Kazan (Volga Region) Federal University, Kazan«Biomedtech KFU» Ltd, Kazan

Kazan (Volga Region) Federal University, Kazan«Biomedtech KFU» Ltd, Kazan

I I Shakhmaeva

Kazan (Volga Region) Federal University, Kazan«Biomedtech KFU» Ltd, Kazan

Kazan (Volga Region) Federal University, Kazan«Biomedtech KFU» Ltd, Kazan

O V Bondar

Kazan (Volga Region) Federal University, Kazan«Biomedtech KFU» Ltd, Kazan

Kazan (Volga Region) Federal University, Kazan«Biomedtech KFU» Ltd, Kazan

T I Abdullin

Kazan (Volga Region) Federal University, Kazan«Biomedtech KFU» Ltd, Kazan

Kazan (Volga Region) Federal University, Kazan«Biomedtech KFU» Ltd, Kazan

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