An in vivo study of pha matrices of different chemical composition: tissue reaction and biodegradation
- Authors: Shishatskaya EI1, Nikolaeva ED2, Goreva AV2, Brigham CJ3, Volova TG1, Sinskey AJ3
-
Affiliations:
- Institute of modern biology and biotechnology, Siberian Federal University, Krasnoyarsk, Russia
- Institute of Biophysics SB RAS, Krasnoyarsk, Russia
- Massachusetts Institute of Technology, Cambridge, USA
- Issue: Vol 7, No 1 (2012)
- Pages: 73-80
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121689
- DOI: https://doi.org/10.23868/gc121689
- ID: 121689
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Abstract
The study addresses consequences of subcutaneous
implantation of film matrices prepared from different PHAs
to laboratory animals. No negative effects of subcutaneous
implantation of PHA matrices on physiological and biochemical
characteristics of the animals were determined. Independently
of the matrices composition and duration of the contact with
the internal environment of the organism we did not observe
any deviations in the behavior of animals, their growth and
development, as well as blood functions. Response of the
tissues to PHA matrices was comparable with the response to
polylactide, but substantially less expressed at the earlier time
periods after implantation. Tissues response to implantation of
PHA of all types is characterized by short-term (up to 2 weeks)
post-traumatic inflammation with formation of fibrous capsules
by 30th-60th days with the thickness less than 100 microns,
which get thinner down to 40-60 microns by 180th day as the
result of involution. No differences in response of tissues and
the whole organism were observed for the matrices produced
from the homopolymer of 3-hydroxybutyric acid (P3HB),
copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids
(P3HB/4HB), 3-hydroxybutyric acid and 3-hydroxyvalerianic
acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoate
acids (P3HB/3HH). Macrophages and foreign-body giant cells
actively participate in the response of the tissues to PHAs. In
the studied conditions matrices from the copolymers containing
3-hydroxyhexanoate and 4 hydroxybutyrate were determined as
more actively degraded PHA. The next less degraded matrices
were matrices from the copolymer of P3HB/3HV and the most
resistant were P3HB matrices. The slower degradation of PHA
matrices was accompanied by delayed development of giantcells
response. The studied PHA matrices can be placed in the
following range by their degradation: P3HB/3HH - P3HB/4HB -
P3HB/HV - P3HB.
implantation of film matrices prepared from different PHAs
to laboratory animals. No negative effects of subcutaneous
implantation of PHA matrices on physiological and biochemical
characteristics of the animals were determined. Independently
of the matrices composition and duration of the contact with
the internal environment of the organism we did not observe
any deviations in the behavior of animals, their growth and
development, as well as blood functions. Response of the
tissues to PHA matrices was comparable with the response to
polylactide, but substantially less expressed at the earlier time
periods after implantation. Tissues response to implantation of
PHA of all types is characterized by short-term (up to 2 weeks)
post-traumatic inflammation with formation of fibrous capsules
by 30th-60th days with the thickness less than 100 microns,
which get thinner down to 40-60 microns by 180th day as the
result of involution. No differences in response of tissues and
the whole organism were observed for the matrices produced
from the homopolymer of 3-hydroxybutyric acid (P3HB),
copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids
(P3HB/4HB), 3-hydroxybutyric acid and 3-hydroxyvalerianic
acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoate
acids (P3HB/3HH). Macrophages and foreign-body giant cells
actively participate in the response of the tissues to PHAs. In
the studied conditions matrices from the copolymers containing
3-hydroxyhexanoate and 4 hydroxybutyrate were determined as
more actively degraded PHA. The next less degraded matrices
were matrices from the copolymer of P3HB/3HV and the most
resistant were P3HB matrices. The slower degradation of PHA
matrices was accompanied by delayed development of giantcells
response. The studied PHA matrices can be placed in the
following range by their degradation: P3HB/3HH - P3HB/4HB -
P3HB/HV - P3HB.
About the authors
E I Shishatskaya
Institute of modern biology and biotechnology, Siberian Federal University, Krasnoyarsk, RussiaInstitute of modern biology and biotechnology, Siberian Federal University, Krasnoyarsk, Russia
E D Nikolaeva
Institute of Biophysics SB RAS, Krasnoyarsk, RussiaInstitute of Biophysics SB RAS, Krasnoyarsk, Russia
A V Goreva
Institute of Biophysics SB RAS, Krasnoyarsk, RussiaInstitute of Biophysics SB RAS, Krasnoyarsk, Russia
C J Brigham
Massachusetts Institute of Technology, Cambridge, USAMassachusetts Institute of Technology, Cambridge, USA
T G Volova
Institute of modern biology and biotechnology, Siberian Federal University, Krasnoyarsk, RussiaInstitute of modern biology and biotechnology, Siberian Federal University, Krasnoyarsk, Russia
A J Sinskey
Massachusetts Institute of Technology, Cambridge, USAMassachusetts Institute of Technology, Cambridge, USA
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