Cryopreservation human placental tissue as source of hematopoietic and mesenchymal stem cells
- Authors: Shablii VA1, Kuchma MD1, Kyryk VM2, Onishchenko AN3, Lukash LL4, Lobitseva GS3
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Affiliations:
- Institute of Molecular Biology and Genetics NAS of Ukraine, KievInstitute of Cell Therapy, Kiev
- State Institute of Genetics and Regenerative Medicine of NAM of Ukraine, Kiev
- Institute of Cell Therapy, Kiev
- Institute of Molecular Biology and Genetics NAS of Ukraine, Kiev
- Issue: Vol 7, No 1 (2012)
- Pages: 54-62
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121684
- DOI: https://doi.org/10.23868/gc121684
- ID: 121684
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Abstract
of placental tissue for the reason to obtain viable hematopoietic
progenitor cells and multipotent mesenchymal stromal cells.
It is established, that the relative content of the cells of CD34+/
SSClow/CD45low cells significantly higher in the cryopreserved
placental tissue. Also we first described the differences
expression of CD90 and CD31on the CD34+/CD45low/SSClow
cells of umbilical cord blood and placenta. Clonal analysis of
cells revealed the presence in cryopreserved placental tissues
of precursors of granulocytes, monocytes and erythrocytes
that forming colonies of granulocyte-monocyte, monocytes,
granulocytes and erythrocytes. Also it was obtained cultures
of stromal cells from cryopreserved placental tissues with
immunophenotype CD90+/CD73+/CD105+/HLA-ABClow/CD45-/
CD34-/CD133-/CD14- and adipogenic and osteogenic potential
in vitro.
About the authors
V A Shablii
Institute of Molecular Biology and Genetics NAS of Ukraine, KievInstitute of Cell Therapy, KievInstitute of Molecular Biology and Genetics NAS of Ukraine, KievInstitute of Cell Therapy, Kiev
M D Kuchma
Institute of Molecular Biology and Genetics NAS of Ukraine, KievInstitute of Cell Therapy, KievInstitute of Molecular Biology and Genetics NAS of Ukraine, KievInstitute of Cell Therapy, Kiev
V M Kyryk
State Institute of Genetics and Regenerative Medicine of NAM of Ukraine, KievState Institute of Genetics and Regenerative Medicine of NAM of Ukraine, Kiev
A N Onishchenko
Institute of Cell Therapy, KievInstitute of Cell Therapy, Kiev
L L Lukash
Institute of Molecular Biology and Genetics NAS of Ukraine, KievInstitute of Molecular Biology and Genetics NAS of Ukraine, Kiev
G S Lobitseva
Institute of Cell Therapy, KievInstitute of Cell Therapy, Kiev
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