«Niche - relief» conception for stem cells as a basis of biomimetic approach to boneand hemopoietic tissues engineering



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Abstract

An affect of relief features and quantitative parameters
of model mineral matrix on in vitro structure-functional
status of human lung prenatal stromal cells (HLPSC) and
in vivo remodeling of mice bone/marrow system has been
studied. According to data established, rough (Ra > 2 m)
implants with calcium phosphate micro-arc coatings simulate
three-dimensional state of regenerating bone matrix. Such
surfaces have own structure-functional sites (microregions)
which were named by niche - relief and were necessary
for maturation and differentiation of HLPSC to secreting
osteoblasts. Maximum remodeling of mice bone/marrow
system in heterotopic test in vivo was also observed under
optimal parameters of osteogenic niche in vitro (approximately
43% average index of cellular alkaline phosphatase square
to artificial microregion area). A probable cell-molecular
mechanism of influence experimental 3D-modeling of bone
surface on selection of quiescent or active state by endosteal
niche has been detected. It connects with activating cellular
production of bone matrix components (alkaline phosphatase,
osteocalcin, collagen, calcium phosphates) that dissociate
dimensionally stromal and hemopoietic elements, and with
parallel diminishing TNF secretion in intercellular medium.
The data obtained develop our previous speculations about
existence and sizes of artificial endosteal niche for osteogenic
differentiation of multipotent mesenchimal stromal cells.

About the authors

I A Khlusov

Siberian State Medical University, Tomsk

Siberian State Medical University, Tomsk

N M Shevtsova

Siberian State Medical University, Tomsk

Siberian State Medical University, Tomsk

M Yu Khlusova

Siberian State Medical University, Tomsk

Siberian State Medical University, Tomsk

K V Zaitsev

Tomsk SRI of Balneology and Physiotherapy, Tomsk

Tomsk SRI of Balneology and Physiotherapy, Tomsk

Yu P Sharkeev

Institute of Strength Physics and Materials Science of SB RAS, Tomsk

Institute of Strength Physics and Materials Science of SB RAS, Tomsk

V F Pichugin

Research and Education Center Biocompatible Materials and Bioengineering attached to Tomsk Polytechnic Universityand Siberian State Medical University, Tomsk

Research and Education Center Biocompatible Materials and Bioengineering attached to Tomsk Polytechnic Universityand Siberian State Medical University, Tomsk

E V Legostaeva

Institute of Strength Physics and Materials Science of SB RAS, Tomsk

Institute of Strength Physics and Materials Science of SB RAS, Tomsk

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