Autophagy induction in peripheral blood T-lymphocytes of atopic asthma patients
- Authors: Skibo YV1, Ponomareva AA2, Reshetnikova ID3, Abramova ZI1
-
Affiliations:
- Kazan (Volga Region) Federal University, Kazan
- Kazan Institute of Biochemistry and Biophysics of RAS, Kazan
- Kazan SRI of Epidemiology and Microbiology, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 146-150
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121650
- DOI: https://doi.org/10.23868/gc121650
- ID: 121650
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Abstract
Autophagy is a fundamental process that ensures
the regulation of T-cell homeostasis. In case of apoptosis
induction disruption in the cell it could be single mechanism of
the cell death. Previously was shown inhibition of lymphocyte
apoptosis in patients with bronchial asthma, so the main study
of this work has focused on the study development process
of autophagy in T-lymphocytes of patients with bronchial
asthma. The article presents the main morphological changes
in cells associated with activation of autophagy (formation
autophagosome). In addition to morphological changes in
lymphocytes, we have shown the expression of autophagy
marker protein (LC3B). We found that in T-lymphocytes of
patients with severe asthma are simultaneous activation of
both autophagy and apoptosis, and autophagy is a stimulus
to cell death.
the regulation of T-cell homeostasis. In case of apoptosis
induction disruption in the cell it could be single mechanism of
the cell death. Previously was shown inhibition of lymphocyte
apoptosis in patients with bronchial asthma, so the main study
of this work has focused on the study development process
of autophagy in T-lymphocytes of patients with bronchial
asthma. The article presents the main morphological changes
in cells associated with activation of autophagy (formation
autophagosome). In addition to morphological changes in
lymphocytes, we have shown the expression of autophagy
marker protein (LC3B). We found that in T-lymphocytes of
patients with severe asthma are simultaneous activation of
both autophagy and apoptosis, and autophagy is a stimulus
to cell death.
Keywords
About the authors
Y V Skibo
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
A A Ponomareva
Kazan Institute of Biochemistry and Biophysics of RAS, KazanKazan Institute of Biochemistry and Biophysics of RAS, Kazan
I D Reshetnikova
Kazan SRI of Epidemiology and Microbiology, KazanKazan SRI of Epidemiology and Microbiology, Kazan
Z I Abramova
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
References
- Бростофор Д., Мейл Д., Ройтт А. и др. Иммунология. Мо- сква: Логосфера; 2007.
- Jameson S.C. Maintaining the norm: T-cell homeostasis. Nat. Rev. Immunol. 2002; 2: 547-56.
- Plas D.R., Rathmell J.C., Thompson C.B. Homeostatic control of lymphocyte survival: potential origins and implications. Nat. Immunol. 2002; 3(6): 515-21.
- Blattman J.N., Antia R., Sourdive D.J. et al. Estimating the precursor frequency of naive antigen-specific CD8 T cells. J. Exp. Med. 2002; 195(5): 657-64.
- Badovinac V.P., Harty J.T. Programming, demarcating, and manipulating CD8+ T-cell memory. Immunol. Rev. 2006; 211: 67-80.
- Pua H.H., Dzhagalov I., Chuck M. et al. A critical role for the autophagy gene Atg5 in T cell survival and proliferation. J. Exp. Med. 2007; 204: 25-31.
- Walter M.J., Holtzman M.J. A centennial history of research on asthma pathogenesis. Am. J. Respir. Cell Mol. Biol. 2005; 32: 483-9.
- Бойчук С.В., Мустафин И.Г. Fas-рецептор и его роль при ато- пических заболеваниях. Иммунология 2001; 3: 24-8.
- O'Sullivan S., Cormican L., Burke C.M. Fluticasone induces T cell apoptosis in the bronchial wall of mild to moderate asthmatics. Asthma 2004; 59(8): 657-61.
- Lockshina R.A., Zakerib Z. Apoptosis, autophagy and more. Int. J. Biochem. Cell B. 2004; 36(12): 2405-19.
- Klionsky D.J. The molecular machinery of autophagy: unanswered questions. Cell Science 2005; 118: 7-18.
- Paglin S., Hollister T., Delohery T. et al. A novel response of cancer cells to radiation involves autophagy and formation of acidic vesicles. Cancer Res. 2001; 61: 439-44.
- Kabeya Y., Mizushima N., Ueno T. et al. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO 2000; 19: 5720-8.
- Li C., Capan E., Zhao Y. et al. Autophagy Is Induced in CD4-T Cells and Important for the Growth Factor-Withdrawal Cell Death. Immunology 2006; 177(8): 5163-8.
- Bursch W. The autophagosomal-lysosomal compartment in programmed cell death. Cell Death Differ. 2001; 8(6): 569-81.
- Edinger A.L., Thompson C.B. Death by design: apoptosis, necrosis and autophagy. Current Opinion in Cell Biology 2004; 16: 663-9.
- Скибо Ю.В., Курмаева Н.Ш. Особенности апоптоза лимфо- цитов у больных легкой и тяжелой атопической бронхиальной аст- мой. Практическая медицина 2012. В печати.
- Скибо Ю.В., Курмаева Н.Ш., Цибулькина В.Н. и др. Струк- тура основных популяций лимфоцитов у больных атопической брон- хиальной астмой разной степени тяжести. Практическая медицина 2012. В печати.
- Kroemer G., Jaattela M. Lysosomes and autophagy in cell death control. Nat. Rev. Cancer. 2005; 5: 886-97.
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