Chromatographic purification of plasmid DNA for clinical applications (gene therapy)
- Authors: Romanova JJ1, Salafutdinov II1,2,3, Zamalyutdinova NM1, Rizvanov AA1
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Affiliations:
- Kazan (Volga region) Federal University, Kazan
- Republican Clinical Hospital, Ministry of Health of the Republic of Tatarstan, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 142-145
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121649
- DOI: https://doi.org/10.23868/gc121649
- ID: 121649
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Abstract
therapy protocols it is necessary to develop methods for
purification of highly homogeneous preparations of recombinant
DNA that do not contain contaminants, primarily chromosomal
DNA, bacterial proteins, RNA and endotoxins. In the course
of our study we performed optimization of the purification of
plasmid supercoiled DNA by three chromatographic steps from
an alkaline lysate of bacterial strain of E. coli. We determined
an optimal conditions for alkaline lysis step in order to increase
the yield and minimize the duration of the plasmid purification
by gel filtration.
About the authors
J J Romanova
Kazan (Volga region) Federal University, KazanKazan (Volga region) Federal University, Kazan
I I Salafutdinov
Kazan (Volga region) Federal University, Kazan;Republican Clinical Hospital, Ministry of Health of the Republic of Tatarstan, Kazan;Kazan (Volga region) Federal University, Kazan;Republican Clinical Hospital, Ministry of Health of the Republic of Tatarstan, Kazan;
N M Zamalyutdinova
Kazan (Volga region) Federal University, KazanKazan (Volga region) Federal University, Kazan
A A Rizvanov
Kazan (Volga region) Federal University, KazanKazan (Volga region) Federal University, Kazan
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