Survival and differentiation of endogenous Schwann cells migrating into spinal cordunder the influence of neurotrophic factors
- Authors: Mukhamedshina YO1,2,3, Shaymardanova GF4, Muhitov AR4, Salafutdinov II2, Zarubina VN1, Rizvanov АА2, Chelyshev Y.A1,2,3
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Affiliations:
- Кazan State Medical University, Kazan
- Кazan (Volga region) Federal University, Kazan
- Kazan Institute of Biochemistry and Biophysics of RAS, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 125-129
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121635
- DOI: https://doi.org/10.23868/gc121635
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Full Text
Abstract
regeneration in the peripheral nervous system. They migrate
into the injury region of spinal cord, which are involved in
remyelination and are regarded as the source of numerous
molecular signals that could potentially support the growth
of axons in the central nervous system. In the present work
we describe the behavior of migrating into the injury dosed
region spinal cord Schwann cells under the influence of
neurotrophic factors - vascular endothelial growth factor
(VEGF) and fibroblast growth factor 2 (FGF2), delivered
by direct introduction of «naked» plasmid DNA and by
transplantation of genetically modified human umbilical cord
blood mononuclear cells.
Using immunohistochemical detection of markers of S100,
GFAP, Krox20 and HSP25 identified different phenotypes
of migrating into the spinal cord of endogenous Schwann
cells. Found that greatest influence on their numbers in the
injury region provides local delivery of genes vegf and fgf2
by human umbilical cord blood mononuclear cells. However,
the direct introduction of the same plasmid may also be
promising in the case of synthetic platforms that enhance
its transfection activity.
Keywords
About the authors
Y O Mukhamedshina
Кazan State Medical University, Kazan;Кazan (Volga region) Federal University, Kazan;Кazan State Medical University, Kazan;Кazan (Volga region) Federal University, Kazan;
G F Shaymardanova
Kazan Institute of Biochemistry and Biophysics of RAS, KazanKazan Institute of Biochemistry and Biophysics of RAS, Kazan
A R Muhitov
Kazan Institute of Biochemistry and Biophysics of RAS, KazanKazan Institute of Biochemistry and Biophysics of RAS, Kazan
I I Salafutdinov
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
V N Zarubina
Кazan State Medical University, KazanКazan State Medical University, Kazan
А А Rizvanov
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
Yu A Chelyshev
Кazan State Medical University, Kazan;Кazan (Volga region) Federal University, Kazan;Кazan State Medical University, Kazan;Кazan (Volga region) Federal University, Kazan;
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