Isolation and cultivation of myofibroblasts from rats liver using explantation method
- Authors: Mijanovic О1, Shafigullina АК2, Rizvanov АА1, Kiasov АP1,2,3
-
Affiliations:
- Kazan (Volga Region) Federal University, Kazan
- Kazan Sate Medical University, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 112-115
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121632
- DOI: https://doi.org/10.23868/gc121632
- ID: 121632
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Abstract
During liver fibrosis development connective tissue
is produced by myofibroblasts that could originate from
two hepatic populations: hepatic stellate cells and portal
fibroblasts. A marker of myofibroblasts is the expression
of -smooth muscle actin (-SMA). Distinctive feature of
myofibroblasts, derived from hepatic stellate cells, is the
preservation of the hepatic stellate cells marker expression -
desmin. The processes of activation, proliferation and cells
trans-differentiation into myofibroblasts are closely related
to the activity of transcription factor NF-kB and its inhibitor
IkB. The aim of our work was to obtain a culture of hepatic
myofibrobasts, to study their origin, phenotype, relations
between NF-kB and IkB expression and the processes of
activation and cells trans-differentiation into myofibroblasts.
For this purpose we isolated heterogeneous population of
cells from rat liver by the method of explantation. Almost
all the cells had desmin and -SMA expression. On this
basis, we suppose that these myofibroblasts were hepatic
stellate cells derivatives, and singular desmin-negative cells
originated from portal fibroblasts. Thus, hepatic stellate cells
have major potential to activation, growth, proliferation and
transdifferentiation into myofibroblasts in comparison to
portal fibroblasts. Activated state of the cells was confirmed
by stable expression of NF-kB and its inhibitor IkB in all the
cells throughout the whole experiment.
is produced by myofibroblasts that could originate from
two hepatic populations: hepatic stellate cells and portal
fibroblasts. A marker of myofibroblasts is the expression
of -smooth muscle actin (-SMA). Distinctive feature of
myofibroblasts, derived from hepatic stellate cells, is the
preservation of the hepatic stellate cells marker expression -
desmin. The processes of activation, proliferation and cells
trans-differentiation into myofibroblasts are closely related
to the activity of transcription factor NF-kB and its inhibitor
IkB. The aim of our work was to obtain a culture of hepatic
myofibrobasts, to study their origin, phenotype, relations
between NF-kB and IkB expression and the processes of
activation and cells trans-differentiation into myofibroblasts.
For this purpose we isolated heterogeneous population of
cells from rat liver by the method of explantation. Almost
all the cells had desmin and -SMA expression. On this
basis, we suppose that these myofibroblasts were hepatic
stellate cells derivatives, and singular desmin-negative cells
originated from portal fibroblasts. Thus, hepatic stellate cells
have major potential to activation, growth, proliferation and
transdifferentiation into myofibroblasts in comparison to
portal fibroblasts. Activated state of the cells was confirmed
by stable expression of NF-kB and its inhibitor IkB in all the
cells throughout the whole experiment.
About the authors
О Mijanovic
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
А К Shafigullina
Kazan Sate Medical University, KazanKazan Sate Medical University, Kazan
А А Rizvanov
Kazan (Volga Region) Federal University, KazanKazan (Volga Region) Federal University, Kazan
А P Kiasov
Kazan (Volga Region) Federal University, Kazan;Kazan Sate Medical University, Kazan;Kazan (Volga Region) Federal University, Kazan;Kazan Sate Medical University, Kazan;
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