Effect of binase on phorbol myristate acetate-induced apoptosis of human peripheral bloodgranulocytes and monocytes
- Authors: Mironov VA1, Shirshikov FV1, Kalacheva NV1, Cherepnev GV2
-
Affiliations:
- Kazan (Volga region) State University, Kazan
- Kazan State Medical Academy, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 108-111
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121631
- DOI: https://doi.org/10.23868/gc121631
- ID: 121631
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Abstract
Effect of binase (RNAse of Bacillus intermedius) on
phorbol myristate acetate-(PMA)-induced apoptosis of human
peripheral blood granulocytes and monocytes was studied in
vitro by flow cytometry. Both toxic (400 μg/ml) and nontoxic
(40 μg/ml) binase concentrations were tested. The binase
end-point effect was dependent on the target cell population
and the binase concentration. In a granulocyte subset, the
400 μg/ml concentration resulted in strongly pronounced
stimulation of PMA-induced apoptosis. In a monocyte subset,
the 40 μg/ml concentration developed a protective effect as
judged by an increase in a percantage of viable cell subset and
by slowing-down cells transtion from an early to late PMAinduced
apoptotic phase.
phorbol myristate acetate-(PMA)-induced apoptosis of human
peripheral blood granulocytes and monocytes was studied in
vitro by flow cytometry. Both toxic (400 μg/ml) and nontoxic
(40 μg/ml) binase concentrations were tested. The binase
end-point effect was dependent on the target cell population
and the binase concentration. In a granulocyte subset, the
400 μg/ml concentration resulted in strongly pronounced
stimulation of PMA-induced apoptosis. In a monocyte subset,
the 40 μg/ml concentration developed a protective effect as
judged by an increase in a percantage of viable cell subset and
by slowing-down cells transtion from an early to late PMAinduced
apoptotic phase.
Keywords
About the authors
V A Mironov
Kazan (Volga region) State University, KazanKazan (Volga region) State University, Kazan
F V Shirshikov
Kazan (Volga region) State University, KazanKazan (Volga region) State University, Kazan
N V Kalacheva
Kazan (Volga region) State University, KazanKazan (Volga region) State University, Kazan
G V Cherepnev
Kazan State Medical Academy, KazanKazan State Medical Academy, Kazan
References
- Kinloch R.A., Theherne J.M., Furness L.M. The pharmacology of apoptosis. Trends in Pharmacological Sciences 1999; 20: 35-42.
- Cameron R.G., Feuer G. Apoptosis and its modulation by Drugs. Heidelberg: Spinger-Verlag; 2000.
- Chandrashekhar Y., Sen S. Gene therapy and pharmaceutical modulation of apoptosis. Cardiol. Clin. 2001; 19: 173-90.
- Makarov A.A., Kolchinsky A., Ilinskaya O.N. Binase and other microbial RNases as potential anticancer agents. BioEssays 2008; 30(8): 781-90.
- Миронов В.А., Ширшиков Ф.В., Калачева Н.В и др. Из- бирательное действие биназы на апоптоз субпопуляций лейкоци- тов крови человека. Фундаментальные исследования 2012; 9(1): 53-9.
- Голубенко И. А., Балабан Н.П., Лещинская И.Б. Рибонуклеаза Bacillus intermedius7P. Очистка хроматографией на фосфоцеллю- лозе и некоторые характеристики гомогенного фермента. Биохимия 1979; 44: 640-8.
- Mower D.A., Peckhman D.W. Decreased membrane phospholipid packing and decreased cell size preceede DNA cleavage in mature B cell apoptosis. J.Immunol. 1994; 152: 4832-48.
- Mussashi M., Ota S, Shiroshita N. The role of protein kinase C isoforms in cell proliferation and apoptosis. J.Hematol. 2000; 72(1): 12-9.
- Миронов В.А., Ширшиков Ф.В., Калачева Н.В и др. Избира- тельное действие биназы на апоптоз субпопуляций лейкоцитов кро- ви человека. Фундаментальные исследования 2012; 9(1): 53-9.
- Fang Е.F., Ng T.B. Ribonucleases of different origins with a wide spectrum of medicinal applications. Biochim. Biophys. Acta . 2011; 1815(1): 65-74.
- Шкляева О.А., Миронова Н.Л., Малкова Е.М. и др. Онко- супрессивное действие РНКазы А и ДНКазы I. Докл. РАН. 2008; 420(1): 134-8.
- Миронов В.А., Филиппов А.В., Ширшиков Ф.В. и др. Влия- ние биназы на некроз и апоптоз макрофагов в модели оксидативно- го стресса. Ученые записки Казанского Университета. В печати.
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