Effect of binase on phorbol myristate acetate-induced apoptosis of human peripheral bloodgranulocytes and monocytes
- Authors: Mironov VA1, Shirshikov FV1, Kalacheva NV1, Cherepnev GV2
-
Affiliations:
- Kazan (Volga region) State University, Kazan
- Kazan State Medical Academy, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 108-111
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121631
- DOI: https://doi.org/10.23868/gc121631
- ID: 121631
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Abstract
Effect of binase (RNAse of Bacillus intermedius) on
phorbol myristate acetate-(PMA)-induced apoptosis of human
peripheral blood granulocytes and monocytes was studied in
vitro by flow cytometry. Both toxic (400 μg/ml) and nontoxic
(40 μg/ml) binase concentrations were tested. The binase
end-point effect was dependent on the target cell population
and the binase concentration. In a granulocyte subset, the
400 μg/ml concentration resulted in strongly pronounced
stimulation of PMA-induced apoptosis. In a monocyte subset,
the 40 μg/ml concentration developed a protective effect as
judged by an increase in a percantage of viable cell subset and
by slowing-down cells transtion from an early to late PMAinduced
apoptotic phase.
phorbol myristate acetate-(PMA)-induced apoptosis of human
peripheral blood granulocytes and monocytes was studied in
vitro by flow cytometry. Both toxic (400 μg/ml) and nontoxic
(40 μg/ml) binase concentrations were tested. The binase
end-point effect was dependent on the target cell population
and the binase concentration. In a granulocyte subset, the
400 μg/ml concentration resulted in strongly pronounced
stimulation of PMA-induced apoptosis. In a monocyte subset,
the 40 μg/ml concentration developed a protective effect as
judged by an increase in a percantage of viable cell subset and
by slowing-down cells transtion from an early to late PMAinduced
apoptotic phase.
Keywords
About the authors
V A Mironov
Kazan (Volga region) State University, KazanKazan (Volga region) State University, Kazan
F V Shirshikov
Kazan (Volga region) State University, KazanKazan (Volga region) State University, Kazan
N V Kalacheva
Kazan (Volga region) State University, KazanKazan (Volga region) State University, Kazan
G V Cherepnev
Kazan State Medical Academy, KazanKazan State Medical Academy, Kazan
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