The action of bacterial serine proteases on cultured animal cell lines
- Authors: Kirillova, JM1, Danilova, JI1, Sharipova MR1
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Affiliations:
- Kazan (Volga region) Federal University, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 88-91
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121607
- DOI: https://doi.org/10.23868/gc121607
- ID: 121607
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Abstract
Our research is direct to determination of serine
proteases (subtilisin and glutamyl endopeptidase) action at
establish cell lines. We determined that the serine proteases
of bacillus are capable cytotoxic action at establish cell lines
of animals. The cell lines are differentiating by sensitivity to
proteins. The cell lines LEK and NGUK were more sensitive
to subtilisin, and line Vero - glutamyl endopeptidase.
proteases (subtilisin and glutamyl endopeptidase) action at
establish cell lines. We determined that the serine proteases
of bacillus are capable cytotoxic action at establish cell lines
of animals. The cell lines are differentiating by sensitivity to
proteins. The cell lines LEK and NGUK were more sensitive
to subtilisin, and line Vero - glutamyl endopeptidase.
About the authors
J M Kirillova,
Kazan (Volga region) Federal University, KazanKazan (Volga region) Federal University, Kazan
J I Danilova,
Kazan (Volga region) Federal University, KazanKazan (Volga region) Federal University, Kazan
M R Sharipova
Kazan (Volga region) Federal University, KazanKazan (Volga region) Federal University, Kazan
References
- Chevance F.F., Hughes K.T. Coordinating assembly of a bacterial macromolecular machine. Nat. Rev. Microbiol. 2008; 6: 455-65.
- Батомункуева Б.П., Егоров Н.С. Изучение препарата внекле- точных протеиназ грибов Aspergillus ochraceus 513 и Aspergillus alliaceus 7 dN 1 Микробиология 2001; 6.
- Шарипова М. Р., Шакиров Е.В., Балабан Н.П. и др. Локали- зация протеолитических ферментов в клетках Bacillus intermedius. Микробиология 2000; 69(5): 660-7.
- Sharipova M.R., Shagimardanova E.I., Chastukhina I.B. et al. The expression of Bacillus intermedius glutamyl endopeptidase gene in Bacillus subtilis recombinant. Mol. Biol. Rep. 2007; 34: 79-87.
- Sharipova M.R., Balaban N.P, Kayumov A.R. et al.The expression of the serine proteinase gene of Bacillus intermedius in Bacillus subtilis. Microbiological Research 2008; 163: 39-50.
- Михайлова Е.О. Субтилизинподобная протеиназа Bacillus intermedius, секретируемая рекомбинантным штаммом Bacillus subtilis на разных фазах роста [диссертация]. Казанский (Приволж- ский) федеральный: Казань; 2007.
- Шамсутдинов Т.Р. Глутамилэндопептидаза Bacillus intermedius, секретируемая рекомбинантным штаммом Bacillus subtilis на разных фазах роста [диссертация]. Казанский (Приволжский) федеральный: Казань; 2009.
- Адамс Р. Методы культуры клеток для биохимиков. М.: Мир; 1983.
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