Methods of cells labeling for visualization in vivo

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Abstract

Today cell transplantation is promising treatment of various diseases. The therapeutic potential of cells depends on their migratory activity, the study of which is necessary for developing effective approaches of cell therapy. There are different approaches to investigate cells survival in vivo and spatial distribution in the organism after transplantation. This review summarizes the comparative characteristics of contrast agents such as fluorescent dyes, nanoparticles, radioisotope, reporter genes, and the features of their interaction with the cells. The main advantages and disadvantages of different methods of labeling cells have been analyzed.

About the authors

A. O Solovieva

Scientific Research Institute of Clinical and Experimental Lymphology of SB RAMS, Novosibirsk; State Research Center of Virology and Biotechnology "Vector”, Koltsovo; Novosibirsk scientific research institute of circulation pathology n.a. academician. E. N. Meshalkin, Novosibirsk

K. E Zubareva

Scientific Research Institute of Clinical and Experimental Lymphology of SB RAMS, Novosibirsk; State Research Center of Virology and Biotechnology "Vector”, Koltsovo

A. F Poveschenko

Scientific Research Institute of Clinical and Experimental Lymphology of SB RAMS, Novosibirsk; Novosibirsk scientific research institute of circulation pathology n.a. academician. E. N. Meshalkin, Novosibirsk

E. A Nechaeva

State Research Center of Virology and Biotechnology "Vector”, Koltsovo

V. I Konenkova

Scientific Research Institute of Clinical and Experimental Lymphology of SB RAMS, Novosibirsk; Novosibirsk scientific research institute of circulation pathology n.a. academician. E. N. Meshalkin, Novosibirsk

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