Antitumor RNase (binase) induces the alteration of cellular permeability

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Abstract

Some RNases including ones of microbial origin possess
antitumor activity, which mechanisms remains unclear. Here
we investigated the first step of RNase action towards
eukaryotic cells which is connected with increase of cell
permeability for ions and macromolecules.
Using radiological analysis of 45Са2+uptake by Candida
yeast and fluorescence imaging of human embryo kidney
cells HEK stained by Ca2+-specific Fura-2/АМ day the level
of intracellular Ca2+ under treatment with the RNase of
Bacillus intermedius (binase) was studied. Viability of lung
carcinoma epithelial cells A549 treated by binase was
measured by WST proliferation kit, stability of erythrocytes
was tested by lysis assay.
We have shown that binase induces the permeability
increase of lower and higher eukaryotic cells for Ca2+as
well as the increase of protein permeability of A549 cells.
Binase treatment protects erythrocytes from osmotic
shock.
The protective or cytotoxic binase effect followed by
increase of cellular permeability is realized depending on the
dell type, where the expression of КСa channels and of certain
oncogens, particularly of ras family, is crucial. The obtained
data supports the significance of the cell permeability increase
as a primary step in the mechanisms of binase-induced
biological effects.

About the authors

H A Cabrera-Fuentes

Kazan (Volga-Region) Federal University, Kazan

Kazan (Volga-Region) Federal University, Kazan

P V Zelenikhin,

Kazan (Volga-Region) Federal University, Kazan

Kazan (Volga-Region) Federal University, Kazan

A I Kolpakov,

Kazan (Volga-Region) Federal University, Kazan

Kazan (Volga-Region) Federal University, Kazan

O N Ilinskaya

Kazan (Volga-Region) Federal University, Kazan

Kazan (Volga-Region) Federal University, Kazan

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