Significance of human herpesvirus detection in multipotent mesenchymal stromal stem cells for clinical practice

The main requirement for the quality of cellular material for clinical use is to ensure that viral control in order to prevent infection of the recipient. The aim of the study was to evaluate the persistence and multiplication of human Herpesviridae in human bone marrow and placenta- derived multipotent mesenchymal stromal cells (MMSCs- BM and MMSCs-P), and their prevalence among donors MMSCs. Expansion MSCs-BM was held from of 6 donors and expansion MMSCs-P was held from of 25 donors for further clinical application of the standard method. We investigated the expression levels of cell surface markers of MMSCs. The quantitative detection of DNA viruses HHV-1, HHV-2, EBV, CMV, HHV-6 was investigated by PCR to the expansion and achieving a sufficient number of MMSCs for clinical use (2-5 passage). In 2 the bone marrow donors was found DNA EBV and HHV-6. These donors were diagnosed lymphohistiocytosis and biliary cirrhosis. In the placenta donors and MMSCs-P were detected DNA EBV and HHV-6 in 2 cases. DNA CMV, HHV-1 HHV-2 and were not detected in any of the investigated samples. The results showed that careful selection of donors, the algorithm reduces the risk of infection of MMSC, the risk of infection with samples of bone marrow donors is increased if the donor bone marrow are patients with severe chronic diseases; EBV and HHV-6 have a tropism to the MMSCs and are able to multiply in the process of expansion; PCR-investigation on the human Herpesviridae before expansion of cells and when a sufficient number of cells MMSCs for further clinical application allows you to quickly identify infected cell material, evaluation of the quality of MMSCs for further clinical application must include the compulsory study of PCR for the human Herpesviridae.

герпесвирусы человека, мультипотентные мезенхимные стромальные клетки, костный мозг, плацента, ПЦР-исследование