Transfer of recombinant nucleic acids into cells (transfection) by means of histones and other nuclear proteins
- Authors: Solovieva VV1, Kudryashova NV1, Rizvanov AA2
-
Affiliations:
- Kazan (Volga Region) Federal university, Kazan
- Kazan (Volga Region) Federal university, KazanKazan State Medical university, KazanRepublican Clinical Hospital of the Health Ministry of the Republic of Tatarstan, Kazan
- Issue: Vol 6, No 3 (2011)
- Pages: 29-40
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121547
- DOI: https://doi.org/10.23868/gc121547
- ID: 121547
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Abstract
Currently a protein/peptide-mediated gene delivery has
been considered a promising approach in non-viral gene
transfer. The previous investigations have shown that histones
and other nuclear proteins might be effective vectors for gene
transfer into cells. Transfection of eukaryotic cells by nucleic
acid and histone complexes (histonefection) effectively occurs
with various histone proteins. The presence of DNA-binding
domains and specific signal sequences of nuclear location
allows to use histones (Н1/Н5, Н2А, Н2В, Н3, Н4) and other
nuclear proteins (such as HMG family proteins and histonelike
prokaryotic proteins) for recombinant genes transfer.
The positive charge of histone protein molecules enables
electrostatic interaction with negatively charged molecules
of nucleic acids and charge neutralization that facilitates
the complexes penetration through a negatively charged
cell membrane. Thus, histonefection is a promising method
for non-viral transfer of recombinant nucleic acids in gene
therapy.
been considered a promising approach in non-viral gene
transfer. The previous investigations have shown that histones
and other nuclear proteins might be effective vectors for gene
transfer into cells. Transfection of eukaryotic cells by nucleic
acid and histone complexes (histonefection) effectively occurs
with various histone proteins. The presence of DNA-binding
domains and specific signal sequences of nuclear location
allows to use histones (Н1/Н5, Н2А, Н2В, Н3, Н4) and other
nuclear proteins (such as HMG family proteins and histonelike
prokaryotic proteins) for recombinant genes transfer.
The positive charge of histone protein molecules enables
electrostatic interaction with negatively charged molecules
of nucleic acids and charge neutralization that facilitates
the complexes penetration through a negatively charged
cell membrane. Thus, histonefection is a promising method
for non-viral transfer of recombinant nucleic acids in gene
therapy.
About the authors
V V Solovieva
Kazan (Volga Region) Federal university, KazanKazan (Volga Region) Federal university, Kazan
N V Kudryashova
Kazan (Volga Region) Federal university, KazanKazan (Volga Region) Federal university, Kazan
A A Rizvanov
Kazan (Volga Region) Federal university, KazanKazan State Medical university, KazanRepublican Clinical Hospital of the Health Ministry of the Republic of Tatarstan, KazanKazan (Volga Region) Federal university, KazanKazan State Medical university, KazanRepublican Clinical Hospital of the Health Ministry of the Republic of Tatarstan, Kazan
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