Safety and effectiveness evaluation of the injection transplantation method of human pacemaker cardiomyocyte derived from induced pluripotent stem cells

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Arrhythmia is a pathological condition leading to a violation of the frequency, rhythm and sequence of the heart contraction. The cell therapy methods open a new possibility for this group of diseases treatment. This work was aimed to evaluate effectiveness and safeness of cardiomyocytes transplantation in the heart of laboratory animals. In the experiment, two groups of animals were examined: in the first group injection of cardiomyocytes contained in Matrigel was made into the wall of the left ventricle, saline injection was performed in the second group. To reduce the rejection reaction after xenogeneic cell culture transplantation prior to surgery and during the whole observation period, the animals were immunosuppressed by cyclosporin A and prednisolone combination. Electrocardiogram was recorded daily during the postoperative follow-up period to catch the ectopic events. After 5 days from cells transplantation animals were euthanized, the myocardium with transplanted material was taken for histological and immunohistochemical analysis. In the course of the work, it was shown that transplanted cardiomyocytes persist in the heart of experimental animals up to 5 days, but no electrophysiological activity was found. The obtained data indicate the need for additional experimental work with increased immunosuppression, increased time of the cells in the myocardium, an increase in the percentage of pacemaker cells in the used culture.

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A. V Ponomarenko

E.N. Meshalkin National Medical Research Center

E. V Chepeleva

E.N. Meshalkin National Medical Research Center

S. V Pavlova

E.N. Meshalkin National Medical Research Center

A. B Romanov

E.N. Meshalkin National Medical Research Center

A. G Strelnikov

E.N. Meshalkin National Medical Research Center

D. S Sergeevichev

E.N. Meshalkin National Medical Research Center


E. A Pokushalov

E.N. Meshalkin National Medical Research Center


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