Effects of autologous gingiva-derived cells with myogenic potential on regeneration of skeletal muscle


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Abstract

In our recent studies we found for the first time the ability of human multipotent mesenchymal stromal cells (MSCs) derived from alveolar gingiva (alveolar mucosa) to differentiate into myogenic direction. The aim of the present study was to evaluate the effects of autologous gingiva-derived MSCs with myogenic potential on the regeneration of muscular tissue after mechanical damage. The study was conducted on 11 male rabbits. Biopsy of alveolar gingiva was performed at each animal before experiment for autologous MSCs obtainment. Cultures of MSCs were induced in vitro into myogenic direction. To model the damage, the medial heads of the gastrocnemius muscles were intersected on both pelvic limbs of the rabbit. Injection of autologous MSCs was performed on the seventh day after injury into the damaged muscle of one of the extremities, while equal volume of saline (control) was injected into the muscle of the contralateral limb. The animals were sacrificed on 0, 21, and 35 days after the administration of cells. MSCs transplantation led to significant reduction of the area of muscle damage. Immunohistochemical analysis revealed earlier increase in the proportion of MyoD- and myogenin-positive cells, as well as decrease in the expression of Ki-67 in damaged tissue, in experimental group compared to the control. Autologous cells did not significantly affect the composition of muscle fibers. Significant decrease in the proportion of fibrous tissue was also observed in the experimental group. The results indicate the effectiveness of autologous alveolar gingiva-derived MSCs for treatment of mechanical damage of muscle tissue. Local administration of cells accelerated reparative regeneration and prevented fibrosis.

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About the authors

I. N Korsakov

Central Clinical Hospital with Outpatient Health Center, Moscow

Email: ikorsakov@yandex.ru

D. P Samchuk

Central Clinical Hospital with Outpatient Health Center, Moscow

A. A Pulin

Central Clinical Hospital with Outpatient Health Center, Moscow

M. O Mavlikeev

Kazan (Volga region) Federal University

O. N Chernova

Kazan (Volga region) Federal University

A. A Titova

Kazan (Volga region) Federal University

R. V Deev

Central Clinical Hospital with Outpatient Health Center, Moscow; Human Stem Cells Institute; I.P. Pavlov Ryazan State Medical University named after academician

I. Y Bozo

Human Stem Cells Institute; A.I. Burnasyan Federal Medical Biophysical Center of FMBA of Russia

V. L Zorin

Central Clinical Hospital with Outpatient Health Center, Moscow; Human Stem Cells Institute

I. I Eremin

Central Clinical Hospital with Outpatient Health Center, Moscow

O. V Denisova

Professional Development Institute of Federal Medical Biological Agency of Russia

A. S Karpukhina

“DiaLab plus”Co. Ltd

A. Yu Gorodkov

A.N. Bakulev Scientific Center for Cardiovascular Surgery

K. V Kotenko

Central State Medical Academy, Moscow

P. B Kopnin

N.N. Blokhin Cancer Research Center

References

  1. McCullagh К., Perlingeiro R. Coaxing stem cells for skeletal muscle repair. Adv. DrugDeliv. Rev. 2015; 84: 198-207.
  2. Деев Р.В., Мавликеев М.О., Бозо И.Я. и др. Генно-клеточная терапия наследственных заболеваний мышечной системы: современное состояние вопроса. Гены и клетки 2014, 9(4): 6-33.
  3. Farini A., Razini P., Erratico S. et al. Cell based therapy for Duchenne muscular dystrophy. J. Cell. Physiol. 2009; 221: 526-34.
  4. Chamberlain J. Gene therapy of muscular dystrophy. Hum. Mol. Genet. 2002; 11: 2355-62.
  5. Long C., McAnally J., Shelton J. et al. Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA. Science 2014; 345t6201):1184-8.
  6. Shi X., Garry D.J. Muscle stem cells in development, regeneration, and disease. Genes and Development 2006; 20t13):1692-708.
  7. Buckingham M., Bajard L., Chang T. et al. The formation of skeletal muscle: from somite to limb. J. Anat. 2003; 202: 59-68.
  8. Meregalli M., Farini A., Sitziaand G.et al. Advancements in stem cells treatment of skeletal muscle wasting. Pathophysiology of skeletal muscle 2014; 5:1-12.
  9. Zammit P., Partridge Т., Yablonka-Reuveni Z. The skeletal muscle satellite cell: The stem cell that came in from the cold. J. of Histochemistry and Cytochem. 2006; 54: 1177-91.
  10. Корсаков И.Н., Зорин В.Л., Еремин И.И. и др. Клеточные технологии для восстановления мышечной ткани, перспективы развития. Часть I: миокард. Гены и клетки 2014; 9(3): 11-7.
  11. Корсаков И.Н., Зорин В.Л., Еремин И.И. и др. Клеточные технологии для восстановления мышечной ткани, перспективы развития. Часть II: скелетные и гладкие мышцы. Гены и клетки 2014; 9(3): 168-72.
  12. Зорин В.Л., Зорина А.И., Гринаковская О.С. и др. Возможности клинического применения клеток с миогенным потенциалом. Клиническая и экспериментальная морфология 2016; 1: 52-62.
  13. Зорин В.Л., Еремин И.И., Рыбко В.А. и др. Слизистая оболочка полости рта - новый источник получения миобластов. Гены и клетки 2014; 9(3): 5-13.
  14. Zorin V.L., Pulin A.A., Eremin I.I. et al. Myogenic potential of human alveolar mucosa derived cells. Cell Cycle 2017; 16(6): 545-55.
  15. Самчук Д.П., Пулин А.А., Еремин И.И. и др. Сравнительный анализ секреторного профиля мезенхимальных стромальных клеток десны человека, дифференцированных в миогенном направлении. Гены и клетки 2015; 10(3): 94-105.
  16. Самчук Д.П., Лукьянова Е.Н., Еремин И.И. и др. Экспрессия генов миогенеза клетками слизистой оболочки десны. Гены и клетки 2015; 10(4): 68-77.
  17. Чернова О.Н., Корсаков И.Н., Самчук Д.П. и др. Экспериментальные модели для изучения регенерации поперечно-полосатой скелетной мышечной ткани. Гены и клетки 2015; 10(4): 127-40.

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