Multipotent stromal cells in the treatment of acute liver failure after extended hepatectomy in the experiment


Cite item

Full Text

Abstract

Resection of the liver is the main method of treatment in primary and metastatic liver tumors. Frequent complication after extended hepatectomy is acute liver failure, which occurs in 33.83% of cases and may lead to death. The aim of this study is to determine the therapeutic potential of multipotent mesenchymal stromal cells in the treatment of acute liver failure after extended hepatectomy in animal models (rats). The liver mass (before and after 70% hepatectomy), synthetic function of the liver, the level of liver enzymes were measured. The results showed that transplantation of 2.5 mil. of multipotent mesenchymal stromal cells of bone marrow into the inferior vena cava improves liver function and decrease the level of liver enzymes. We observed better recovery of liver mass, and liver functional test (total protein, albumin, alkaline phosphatase, INR, APTT, direct bilirubin) in the experimental group. Thus, multipotent mesenchymal stromal cells have therapeutic effect in treatment of acute liver failure after extended hepatectomy in the experiment. However, more research is needed to understand the mechanisms of therapeutic action of these cells after extended hepatectomy.

Full Text

Restricted Access

About the authors

V. S Rudakov

Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency

Moscow, Russia

S. E Voskanyan

Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency, Moscow, Russia

Moscow, Russia

I. I Eremin

Central Clinical Hospital with Polyclinics of Administration of President of the Russian Federation

Moscow, Russia

I. E Onnitsev

S.M. Kirov Military Medical Academy

Saint-Petersburg, Russia

R. V Deev

Human Stem Cells Institute; I.P. Pavlov Ryazan State Medical University

Ryazan, Moscow Russia

E. V Naydenov

Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency

Ryazan, Russia

P. S Eremin

Central Clinical Hospital with Polyclinics of Administration of President of the Russian Federation

Ryazan, Russia

1 1 A.A. Y.A. Zhgutov

Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency

Moscow, Russia

References

  1. Schroeder R.A., Marroquin C.E., Bute B.P. et al. Predictive indices of morbidity and mortality after liver resection. Ann. Surg. 2006; 243: 373-9.
  2. Патютко Ю.И., Сагайдак И.В., Котельников А.Г. и др. Резекция печени: современные технологии при опухолевом поражении. Анналы хирургической гепатологии 2010; 15: 9-17.
  3. Mahnken A.H. Current status of transarterial radioembolization. World J. Radiol. 2016; 8: 449-59.
  4. Masayuki I., Toru M., Kohei H. et al. Comprehensive review of post-liver resection surgical complications and a new universal classification and grading system. World J. Hepatol. 2014; 6: 745-51.
  5. Shan J., Quan F., Gerile W. et al. Management of post-hepa-tectomy complications. World J. Gastroenterol. 2013; 19: 7983-91.
  6. Bernal W., Wendon J. Acute liver failure. NEJM 2013; 369: 2525-34.
  7. Knoefel W.T., Alexander A., Tustas R.Y. et al. Stem cell-induced liver regeneration. Zentralbl. Chir. 2013; 138: 166-72.
  8. Vivarelli M., Vincenzi P., Montalti R. et al. ALPPS Procedure for extended liver resections: a single centre experience and a systematic review. PLoS One 2015; 10: 23.
  9. Lihua S., Xiaotang F., Lijuan Z. et al. Bone mesenchymal stem cell transplantation via four routes for the treatment of acute liver failure in rats. Inter. J. Mol. Med. 2014; 34: 987-96.
  10. Liu W., Song F., Ren L. et al. The multiple functional roles of mesenchymal stem cells in participating in treating liver diseases. Cell. Mol. Med. 2015; 3: 511-20.
  11. Котенко К.В., Восканян С.Э., Еремин И.И., и др. Перспективы применения клеточных продуктов в хирургической гепатологии. Гепатогастроэнтерология 2011; 5-6: 208-16.
  12. Deng L., Liu G., Wu X. et al. Adipose derived mesenchymal stem cells efficiently rescue carbon tetrachloride-induced acute liver failure in mouse. Sci. World J. 2014; 1-8.
  13. Alison M.R., Islam S., Lim S. Stem cells in liver regeneration, fibrosis and cancer: the good, the bad and the ugly. Pathol. 2009; 217: 282-98.
  14. Рудаков В.С., Восканян С.Э., Еремин И.И. и др. Экспериментальные модели острой печеночной недостаточности. Российский медико-биологический вестник имени академика И.П. Павлова 2015; 4: 138-44.
  15. Sun X.E., Zhang X.Q., Liu M.M. Effect of bone marrow mwsenchymal stem cells on the TGF-B1/Smad signaking pathway of hepatic stellate. Gen. Mol. Res. 2015; 14: 8744-54.
  16. Tsai C.Y., LinY.S., Yeh T.S. et al. Disrupted hepatic adiponectin signaling impairs liver regeneration of steatotic rats. Chang Gung Med. J. 2011; 34(3): 248-59.
  17. Shufang Y., Tao J., Rongiong Z. et al. Effect of bone marrow mesenchymal stem cell transplantation on acute hepatic failure in rats. Exp. Ther. Med. 2014; 8: 1150-8.
  18. Yilmaz E. D., Motor S., Sefil F. et al. Effects of paliperidone palmitate on coagulation: an experimental study. Sci. World J. 2014; 1-5.
  19. Michalopoulos G.K., Marie C. Liver Regeneration. DeFrances Science 1997; 276: 60-6.
  20. Panpan C., Jiajia C., Chenxia H. et al. Noninvasive in-vivo tracing and imagimg of transplanted stem cells for liver regeneration. Stem Cell Res. Ther. 2016; 7: 1-11.
  21. Akhan E., Tuncel D., Akcali K.C. Nanoparticle labeling of bone marrow-derived rat mesenchymal stem cells: their use in differentiation and tracking. Biomed. Res. Int. 2015; 19: 511-20.
  22. Liu T., Mu H., Shen Z. et al. Autologous adipose tissue-derived mesenchymal stem cells are involved in rat liver regeneration following repeat partial hepatectomy. Mol. Med. Rep. 2016; 13: 2053-9.
  23. Kharaziha P., Hellstrom P.M., Noorinayer B. et al. Improvement of liver function in liver cirrhosis patients after autologous mesenchymal stem cell injection: a phase I-II. Eur. J. Gastroenterol Hepatology 2009; 21: 1199-205.
  24. Jiamin Z., Shiyuan Z., Yi Z. et al. Hepatocyte growth factor gene-modified adipose derived mesenchymal stem cells ameliorate radiation induced liver damage in a rat model. Plos One 2014; 9 (12): 1-20.

Copyright (c) 2016 PJSC Human Stem Cells Institute


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС 77 - 57156 от 11.03.2014.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies