Investigating the properties of membrane vesicles obtained from human cells HEK293 using cytochalasin B
- Authors: Gomzikova M.O1, Rizvanov A.A1
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Affiliations:
- Issue: Vol 10, No 3 (2015)
- Pages: 27-32
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/120467
- DOI: https://doi.org/10.23868/gc120467
- ID: 120467
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Abstract
The preparation method of membrane vesicles from human cells using cytochalasin B allows to overcome the limitations of human cells natural microvesicles, associated with the complex procedure of isolation and limited output. Membrane vesicles (MV) prepared from human cells are a promising vector for delivering of various bioactive substances. We performed the preparation of MV from human cells HEK293 using cytochalasin B and size determination of the MV. Then we studied the influence of applied MV concentration and intravesicular substance concentration on the substance delivery effectiveness to recipient cells. It was found that MV ranging in size from 164.2 nm to 3580 nm, but the most of MV sized from 164.2 nm to 712,4 nm (84. 6%). MV are able to enclose the cytoplasmic contents of the parent cells and deliver it to recipient cells, the amount of delivered substance (CFDA SE) to the recipient cells is proportional to the loaded substances into MV
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About the authors
M. O Gomzikova
Kazan (Volga Region) Federal University Kazan, Russia
A. A Rizvanov
Email: Albert.Rizvanov@kpfu.ru
Kazan (Volga Region) Federal University Kazan, Russia
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