Cytotoxic activity of plant oils of Nigella sativa, salvia officinalis and peptide metabolites of streptomyces mirabilis fK749 against HeLa and MCF-7 cancer cell lines

In the last several years, there has been an increase in the use of plant materials in clinical development of new drugs and methods in the treatment of neoplastic diseases. In this regard, the current study was aimed to investigate the cytotoxic activity of Nigella sativa (NS), Salvia officinalis (SO) plant oils and peptide metabolites (PM) of Streptomyces mirabilis FK749 on the viability of Hela and MCF-7 cancer cells. To achieve this objective, cells were exposed to 0, 20, 40, 60, 80 and 100 mg/ml of NS, SO plant oils and PM S. mirabilis FK749. After 24 h. incubation the effect of plant oils and PM on the viability and apoptotic activity of the cancer cells were assessed by using trypan blue and TMRE assays. Morphological changes were examined. Whereas, the half-maximal inhibitory concentrations of these oils and PM were assessed after 72 h. incubation. The results of this study showed that treatment Hela and MCF-7 cells with NS, SO and PM at 40 mg/ml and above concentrations up to 100 mg/ml were found to be cytotoxic and leads to a decrease in the cell viability. The morphological changes observed in Hela and MCF-7 cells exposed to different concentrations of NS SO and PM were found to be in a concentration dependent manner. Hela cells were shown to be more resistant against oil components and PM than MCF-7 cells.

Nigella sativa, Salvia officinalis, Streptomyces mirabilis, HeLa, MCF-7, Nigella sativa, Salvia officinalis, Streptomyces mirabilis, HeLa, MCF-7, cytotoxic activity