Immunohistochemical study of motoneurons of the lumbar spinal cord of mice after the 30-days space flight on biosatellite BION-M1
- Authors: Tyapkina O.V1,2,3, Rezvyakov P.N2, Nurullin L.F1,3, Petrov K.A1,3, Nikolskiy E.E1,2,3, Islamov R.R1
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Affiliations:
- Kazan State Medical University
- Kazan Institute of Biochemistry and Biophysics of RAS
- Kazan (Volga Region) Federal University
- Issue: Vol 9, No 3 (2014)
- Pages: 263-266
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/120349
- DOI: https://doi.org/10.23868/gc120349
- ID: 120349
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Abstract
We made immunohistochemical study of the lumbar spinal cord of c57black/6 mice after a 30-days space flight using antibodies against proteins which involved in providing of synaptic transmission (synaptophysin and PSD95). Also we use antibodies against proteins of intracellular protection (Hsp25 and Hsp70), and neuroprotective protein (VEGF) and its receptor (Flt-1). We found, that the levels of synaptophysin immune expression decreased by 21%, PSD - by 55%, Hsp25 - by 15% and Hsp70 - by 9% in mice lumbar spinal cord motoneurons after space flight. Thus, there were no significant changes in the immune expression of VEGF and its receptor Flt-1. Obtained data reveal the changes in the functional state (synaptic activity and resistance to stress) of mice lumbar spinal cord motoneurons after space flight. So, we have shown a new data about spinal cord motoneurons role in the development of hypogravity motor syndrome.
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About the authors
O. V Tyapkina
Kazan State Medical University; Kazan Institute of Biochemistry and Biophysics of RAS; Kazan (Volga Region) Federal University
P. N Rezvyakov
Kazan Institute of Biochemistry and Biophysics of RAS
L. F Nurullin
Kazan State Medical University; Kazan (Volga Region) Federal University
K. A Petrov
Kazan State Medical University; Kazan (Volga Region) Federal University
E. E Nikolskiy
Kazan State Medical University; Kazan Institute of Biochemistry and Biophysics of RAS; Kazan (Volga Region) Federal University
R. R Islamov
Kazan State Medical University
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