Histotechnical solutions for quality improvement of nucleic acid specimens extracted from paraffin blocks

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Abstract

With the growing body of information about molecular nature of a set of diseases, the question of molecular biology techniques acceptability for histological material study came up. Currently available pathology archives could provide vast quantity of material for retrospective studies, however, the most frequently used fixative formalin dramatically damages nucleic acids in the tissue. A similar problem arises with the material that come into the pathology department and undergoing conventional histological processing in nowadays. In recent years with the entry into the practice of diagnostic methods based on molecular biology methods it has become especially important to obtain high-quality samples of nucleic acids. Research devoted to development of new approaches of histological material handling from one side and solutions for nucleic acids extraction from tissue after standard histological treatment on the other side revealed a number of ways to overcome the problem of poor quality of nucleic acids isolated from the paraffin block. The aim of this review is to highlight the modifications histological processing techniques that allow prepare nucleic acids samples of higher quality.

About the authors

A. N Vaganova

BioVitrum Ltd, Moscow, Russia

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