The spread of mobile genetic elements SCCmec-type strains Staphylococcus Aureus, isolated with skin and mucous of patients with allergic pathology



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Abstract

Staphylococcus aureus is widespread bacterium that causes inflectional diseases of humans and animals. Currently, there is growing incidence of methicillin-resistant strains of Staphylococcus aureus. Resistance of Staphylococcus aureus strains to methicillin is detected by the presence in the genome of staphylococcal chromosomal cassette of SCCmec type. In this work 60 clinical strains of MRSA isolated from the skin and mucous of patients with allergic pathology were examined. The analysis of Staphylococcus aureus strains was performed in differential mediums. Additionally, specific identification was carried out by MALDI-TOF. Specific regions of the genes of mec gene complex were identified by PCR. As a result of typing we determined that 47% of tested strains were resistant to oxacillin phenotypically, but did not contain the specific primer sites related to mec- gene complex types I-IV. The remaining 53% of the strains were contained primer-specific amplicons. These strains were also phenotypically resistant to oxacillin. This study showed that MRSA strains as part of its genetic apparatus are several different types of SCCmec cassettes. This property allows them to express multidrug resistance.

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About the authors

A. F Shamsutdinov

Kazan (Volga region) Federal University; Research Institute of Epidemiology and Microbiology

A. A Toymentseva

Kazan (Volga region) Federal University

Yu. A Tyurin

Research Institute of Epidemiology and Microbiology; Kazan State Medical University

L. T Bayazitova

Research Institute of Epidemiology and Microbiology

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