Structural dynamics of adhesive bone marrow cells by cultivation: primary passage (part 1)

Structural dynamics of heterogenic adhesive human bone marrow cells was studied using primary cultures of donor's bone marrow cells. Various light microscopic techniques including Hoffman modulation contrast and Nomarski differential interference contrast were used to study cell cultures. Automatic interval photography of cell cultures with constantly maintained focusing under conditions of culture incubator that was integrated with inverted microscope was performed. Four degrees of cell passage density were used. Marked polymorphism of passaged, adhered and subsequently cultured cells was demonstrated. Four groups of adhesive cells with different morphology and structural dynamics were isolated. The majority of adhesive cells showed no marked proliferative activity and only their insignificant part possessed high proliferative potentialities. Several ways for compact monolayer formation depending on primary culture cell passage density were determined. Achieved data broaden the notion of the nature and structural dynamics of human bone marrow adhesive cells in primary culture in vitro.

bone marrow cells, primary cultures, adhesive cells