Different bone marrow cells autotransplantation influence on morphofunctional state of myocardium after experimental infarction

V. V. Davydenko; Давыденко В. В.; A. A. Matyukov; Матюков А. А.; N. V. Tsupkina; Цупкина Н. В.; T. D. Vlasov; Власов Т. Д.; V. V. Gritsenko; Гриценко В. В.; A. A. Kuznetsov; Кузнецов А. А.; Kh. K. Аmineva; Аминева Х. К.; R. V. Deev; Деев Р. В.; A. N. Yalfimov; Ялфимов А. Н.; G. P. Pinaev; Пинаев Г. П.

Different bone marrow cells autotransplantation influence on morphofunctional state of myocardium after experimental infarction

Authors: Davydenko V.V.¹, Matyukov A.A.¹, Tsupkina N.V.², Vlasov T.D.¹, Gritsenko V.V.¹, Kuznetsov A.A.¹, Аmineva K.K.¹, Deev R.V.³, Yalfimov A.N.¹, Pinaev G.P.²
Affiliations:
1. Saint-Petersburg I.P. Pavlov State Medical University
2. Institute of Cytology, RАS
3. Kirov Military Medical Аcademy
Issue: Vol 2, No 2 (2007)
Pages: 52-61
Section: Original Study Articles
URL: https://genescells.ru/2313-1829/article/view/217707
ID: 217707

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Abstract

The contribution aims to compare influence of intramyocardial autotransplantation of bone marrow multipotent mesenchymal stromal cells and bone marrow nucleated cells on a myocardium morphofunctional state after infarction. Chinchilla rabbits were exposed in the experiment. Myocardial infarction was modeled by ligating the anterior descending branch of the left coronary artery. The condition was evaluated with functional (electrocardiography, echocardiography) and morphologic methods. There were three groups of animals. To the first group animals (control, n = 13) α-МЕМ growth medium was injected into the damaged area; a culture of bone marrow multipotent mesenchymal stromal cells (2х10⁶) was introduced to the 2^nd group animals (n = 14), while the animals of the 3^rd group (n = 14) being given bone marrow nucleated cells (2х10⁶). It has been shown that intramyocardial autotransplantation of bone marrow multipotent mesenchymal stromal cells in experimental myocardial infarction resulted in ischemic area restriction, normalization of systolic function indices as well as stimulation of angiogenesis. Аt the same time intramyocardial autotransplantation of bone marrow nucleated cells was followed by an expansion of ischemic area and reduction of systolic function indices as compared with the controls, although angiogenesis activation occurred.

Keywords

myocardial infarction, multipotent mesenchymal stromal cells, nucleated cells, bone marrow, autologous transplantation, electrocardiography, echocardiography

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