Features of interaction between hematopoietic stem and tumor cells of different lines in vitro



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Abstract

One of the promising directions of the brain tumors treatment connected using hematopoietic stem cells (HSC). The aim of this work: to study the features of the interaction between the human stem and tumor cells of different lines. The materials for the study were CD 34+-HSCs, U87 glioblastoma, lung cancer (A549), breast cancer (MCF-7). We used cell culture methods, the robotic monitoring of cellcell interactions, confocal laser microscopy, flow cytometry. It was shown that the mobility of HSCs to glioblastoma cells was higher than to cells of lung and breast cancer. The high mobility of HSCs combined with the ability to adhere to tumor cells and to share with them the contents of the cytoplasm. A direction of transport cytoplasm was depended from the tumor cell types and is a feature of the process. We believe that the described phenomenon can be one of the mechanisms of invasive growth and also indicates a high antitumor potential of HSCs.

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About the authors

E. V Milkina

School of Biomedicine of the Far Eastern Federal University; A.V. Zhirmunski Institute of Marine Biology, Far Eastern Branch of the RAS

Vladivostok, Russia

P. V Mischenko

School of Biomedicine of the Far Eastern Federal University; A.V. Zhirmunski Institute of Marine Biology, Far Eastern Branch of the RAS

Vladivostok, Russia

S. V Zaytsev

School of Biomedicine of the Far Eastern Federal University

Vladivostok, Russia

I. S Bryukhovetskiy

School of Biomedicine of the Far Eastern Federal University;A.V. Zhirmunski Institute of Marine Biology, Far Eastern Branch of the RAS

Vladivostok, Russia

I. V Dyuyzen

School of Biomedicine of the Far Eastern Federal University; A.V. Zhirmunski Institute of Marine Biology, Far Eastern Branch of the RAS

Vladivostok, Russia

A. S Bryukhovetskiy

School of Biomedicine of the Far Eastern Federal University

Vladivostok, Russia

Y. S Khotimchenko

School of Biomedicine of the Far Eastern Federal University; A.V. Zhirmunski Institute of Marine Biology, Far Eastern Branch of the RAS

Vladivostok, Russia

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