Development of tissue-engineered chitosan-polycaprolactone blends for vascular surgery



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Abstract

Tissue engineering provides the opportunity to minimize some possible negative results of the synthetic vascular grafts in long-term follow-up. The choice of the optimal scaffold and cell source for seeding are key conditions to bring properties of vessel substitute to physiological. In some works it is shown that a chitosan-polycaprolactone blend is a suitable biodegradable material for tissue engineering. In this paper we suggest an efficient method to generate of tissue-engineered chitosan-polycaprolactone blends, cellularized by endothelial cells of human cardiac explants. The cells cultured on the blended membranes retain their functional properties: viability and proliferative properties; maintain specific endothelial antigens and synthesis of extracellular matrix. These results suggested that tissue-engineered chitosan-polycaprolactone blends seeded by endothelial cells of human cardiac explants may be potential to development of substitutes for small diameter blood vessels.

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About the authors

I. S Zakharova

E.N. Meshalkin Siberian Federal Biomedical Research Center;Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS; Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the RAS.

Novosibirsk, Russia

A. M Smirnova

Novosibirsk State University;Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS;E.N. Meshalkin Siberian Federal Biomedical Research Center.

Novosibirsk, Russia

M. K Zhiven'

E.N. Meshalkin Siberian Federal Biomedical Research Center;Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS;Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the RAS.

Novosibirsk, Russia

Sh. B Saaya

E.N. Meshalkin Siberian Federal Biomedical Research Center.

Новосибирск, Россия.

A. I Shevchenko

Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS;Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the RAS;E.N. Meshalkin Siberian Federal Biomedical Research Center; Novosibirsk State University.

Novosibirsk, Russia

S. M Zakian

Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS;Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the RAS;E.N. Meshalkin Siberian Federal Biomedical Research Center; Novosibirsk State University.

Novosibirsk, Russia

L. N Ivanova

Novosibirsk State University; Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS.

Novosibirsk, Russia

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