Proliferation and transdifferentiation of endocrinocytes of the rat during experimental diabetes



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Abstract

Some studies have found an increase number of α-cells in experimental diabetes, which may cause rising of blood glucose levels, along with the lack of insulin. But the mechanism of increasing the amount of glucagon-positive cells is still unknown. The aim of the study was to investigate the proliferative activity and the possibility of differentiation of α- and β-cells of the islets of Langerhans of pancreas during experimental diabetes in rats The work was performed on 33 white mongrel male rats. After alloxan injection, blood glucose levels were measured by glucose oxidase method and the expression of insulin, glucagon, and proliferating cell nuclear antigen was studied. Isolated proliferating glucagon-positive cells were found only on day 14 of the experiment. At the same time of the experiment bigormonal cells were found that synthesize insulin and glucagon. The results of the double staining for PCNA and glucagon showed that the increasing number of glucagon-positive cells in early stages of experimental diabetes is not related to their proliferation Probably it is due to differentiation of the progenitor cells of the islets in pancreas

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About the authors

M. S Kaligin

Email: mfkaligin@mail.ru
Kazan (Volga Region) Federal University Kazan, Russia

M. O Mavlikeev

Kazan (Volga Region) Federal University Kazan, Russia

A. A Titova

Kazan (Volga Region) Federal University Kazan, Russia

A. S Plushkina

Kazan (Volga Region) Federal University Kazan State Medical University Kazan, Russia

M. A Titova

Kazan (Volga Region) Federal University Kazan, Russia

A. A Gumerova

Kazan (Volga Region) Federal University Kazan, Russia

A. P Kiassov

Kazan (Volga Region) Federal University Kazan State Medical University Kazan, Russia

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