Hematopoietic stem cells with induced apoptosis effectively inhibit glioma cell growth in vitro, but started new mechanism of tumor stem cells



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Abstract

The presence of hematopoietic stem cells in culture inhibits proliferation of tumor cells. We hypothesized that pretreatment of stem cell apoptosis inducers increase their anti-tumor efficacy. The aim of the study was to explore the possibility of stem cells to induce apoptosis effectively inhibit the growth and induce the death of tumor cells in vitro. Use line C6 glioma cultures and hematopoietic (CD34+/CD45 + ) stem cells. We used dexamethasone as the apoptosis inducer. It's known that hematopoietic stem cells with apoptosis induced more effectively inhibit growth and induce a death of C6 glioma cells than native stem cells. In the process of intercellular interaction we observed phenomenon of merging stem and tumor cells, which apparently is one of the mechanisms of new tumor stem cells formation.

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About the authors

I. S Bryukhovetskiy

Far Eastern Federal University; A.V. Zhirmunsky Institute of Marine Biology of FEB RAS

Vladivostok, Russia

P. V Mischenko

Far Eastern Federal University; A.V. Zhirmunsky Institute of Marine Biology of FEB RAS

Vladivostok, Russia

E. V Tolok

Far Eastern Federal University; A.V. Zhirmunsky Institute of Marine Biology of FEB RAS

Vladivostok, Russia

Yu. S. Khotimchenko

Far Eastern Federal University; A.V. Zhirmunsky Institute of Marine Biology of FEB RAS

Vladivostok, Russia

S. V Zaitsev

Far Eastern Federal University

Vladivostok, Russia

A. S Bryukhovetskiy

Far Eastern Federal University; Clinic of Restorative and Interventional Neurology and Therapy “NeuroVita"

Vladivostok, Russia

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