The effect of severe polytrauma on the migration of hematopoietic stem cells in mice

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Abstract

It has been shown that hematopoietic stem cells (SCCS) are involved in the physiological and reparative regeneration of non-hematopoietic tissues and organs through transdifferentiation and/or cell fusion. In this regard, it seems interesting to evaluate the kinetics of bone marrow CCM in severe trauma. Three experimental models were used in the study: injury to mice followed by uneven irradiation in lethal doses with 1/2 shin screening (A), irradiation of mice in lethal doses followed by transplantation of bone marrow cells from injured mice (B), injury to mice followed by irradiation in sublethal doses (C). The migration of CCM was assessed by registering hematopoietic colonies growing on the spleen of irradiated mice from endo- or exogenous SCCS. In experimental models A and B, it was shown that a significantly larger number of endocolonies are formed in the spleens of injured mice in comparison with mice of control groups. In the experimental model B, it was shown that the bone marrow of injured mice contains a significantly smaller number of CCM capable of migration. Based on the results obtained, it was concluded that severe polytrauma induces CCM migration in mice.

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About the authors

V. N. Alexandrov

S.M. Kirov Military Medical Academy

Author for correspondence.
Email: redaktor@celltranspl.ru
Russian Federation, Saint-Petersburg

V. S. Sergeev

S.M. Kirov Military Medical Academy

Email: redaktor@celltranspl.ru
Russian Federation, Saint-Petersburg

References

  1. Krause D.S., Theise N.D., Collector M.I. et al. Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell. Cell 2001; 105(3): 369-77.
  2. Orlic D., Kajstura J., Chimenti S. et al. Bone marrow cells regenerate infracted myocardium. Nature 2001 ; 410: 701-5.
  3. Wagers A.J., Sherwood R.I., Christensen J.L., Weissman I.L. Little evidence for developmental plasticity of adult hematopoietic stem cells. Science 2002; 297: 2256-9.
  4. Murry C.E., Soonpaa M.H., Reinecke H. Haematopoietic stem cells do not transdifferentiate into cardiac myocytes in myocardial infarcts. Nature 2004; 428: 664-8.
  5. Terada N., Hamazaki T., Oka M. Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion. Nature 2002: 416: 542-54.
  6. Polesskaya A., Seale P., Rudnicki M.A. Wnt Signaling Induces the Myogenic Specification of Resident CD45+ Adult Stem Cells during Muscle Regeneration. Cell 2003; 113: 841-52.
  7. Vassilopoulos G., Wang P.R. Russell D.W. et al. Transplanted bone marrow regenerates liver by cell fusion. Nature 2003; 422: 823-5.
  8. Dekel B., Shezen E., Even-Tov-Friedman S. et al. Transplantation of human CD34+CD133+ hematopoietic stem cells into ischemic and growing kidneys suggests role in vasculogenesis but not tubulogenesis. Stem cells. First published online January 12, 2006.
  9. Lyden D., Hattori K., Dias S. et al. Impaired recruitment of bone-marrow- derived endothelial and hematopoietic precursor cells blocks tumor angiogenesis and growth. Nat. Med. 2001; 11: 1194-201.
  10. Thom S.R., Bhopale V.M. Stem cell mobilization by hyperbaric oxygen. Am. J. Physiol. Heart Circ. Physiol. 2006; 290: 1378-86.
  11. Liles W.C., Rodger E., Broxmeyer H.E. et al. Augmented mobilization and collection of CD34+ hematopoietic cells from normal human volunteers stimulated with granulocyte-colony-stimulating factor by single-dose administration of AMD3100, a CXCR4 antagonist. Transfusion 2005; 45: 295-300.
  12. Takeyama K., Ohto H. PBSC mobilization. Transfus. Apher. Sci. 2004; 31: 233-43.
  13. Dalakas E., Newsome P.N., Harrison D.J., Plevris J.N. Hematopoietic stem cell trafficking in liver injury. The FASEB Journal 2005; 19: 1225-31.
  14. Di Campli C., Piscaglia A.C., Giuliante F. et al. No evidence of hematopoietic stem cell mobilization in patients submitted to hepatectomy or in patients with acute on chronic liver failure. Transplant Proc. 2005; 37: 2563-6.
  15. Till J. E., McCalloch E.A. A direct measurement of the radiation sensitivity of normal bone marrow cells. Radiat. Res. 1961; 14: 213-22.
  16. Till J.E., McCalloch E.A. Early repair in marrow cells irradiated and proliferation in vivo. Radiat. Res. 1963; 18: 96-105.
  17. Abe S., Lauby G., Boyer G. et al. Lung Cells Transplanted to Irradiated Recipients Generate Lymphohematopoietic Progeny. Am. J. Respiratory Cell and Mol. Biol. 2004; 30; 491-9.
  18. Kotton D.N., Fabian A.J., Mulligan R.C. A novel stem-cell population in adult liver with potent hematopoietic-reconstitution activity. Blood 2005; 106: 1574-80.
  19. Jackson K.A., Mi T., Goodell M.A. Hematopoietic potential of stem cells isolated from murine skeletal muscle. Immunology 1999; 96: 14482-6.
  20. Jay K.E., Gallacher L., Bhatia M. Emergence of muscle and neural hematopoiesis in humans. Blood 2002; 100: 3193-202.
  21. McKinney-Freeman S.L., Jackson K.A. Muscle-derived hematopoietic stem cells are hematopoietic in origin. PNAS 2002; 99: 1341 -6.
  22. Issarachai S., Priestley G.V., Nakamoto B. Cells with hemopoietic potential residing in muscle are itinerant bone marrow-derived cells. Exp. Hematol. 2002; 30: 366-73.
  23. Aiuti A.,Webb I.J., Bleul C. et al. The Chemokine SDF-1 Is a Chemoattractant for Human CD34 Hematopoietic Progenitor Cells and Provides a New Mechanism to Explain the Mobilization of CD34 Progenitors to Peripheral Blood. J. Exp. Med. 1997; 185: 111-20.
  24. Juarez J., Bendall L. SDF-1 and CXCR4 in normal and malignant hematopoiesis. Histol. Histopathol. 2004; 19: 299-309.
  25. Askari A.T., Unzek S., Popovic Z.B. et al. Effect of stromal-cell-derived factor 1 on stem-cell homing and tissue regeneration in ischaemic cardiomyopathy. Lancet 2003; 362: 697-703.
  26. Ratajczak M.Z., Majka M., Kucia M. et al. Expression of functional CXCR4 by muscle satellite cells and secretion of SDF-1 by muscle-derived fibroblasts is associated with the presence of both muscle progenitors in bone marrow and hematopoietic stem/progenitor cells in muscles. Stem Cells 2003; 21: 363-71.
  27. Hatch H.M., Zheng D., Jorgensen M.L. et al. SDF-1alpha/CXCR4: a mechanism for hepatic oval cell activation and bone marrow stem cell recruitment to the injured liver of rats. Cloning Stem Cells 2002; 4: 339-51.
  28. Lazarini F., Tham T.N., Casanova P. et al. Role of the alpha-chemokine stromal cell-derived factor (SDF-1) in the developing and mature central nervous system. Glia 2003;42: 139-48.
  29. Schrader A.J., Lechner O., Templin M. et al. CXCR4/CXCL12 expression and signalling in kidney cancer. Br. J. Cancer 2002; 86: 1250-6.
  30. Hattori K., Heissig B., Tashiro K. et al. Plasma elevation of stromal cell- derived factor-1 induces mobilization of mature and immature hematopoietic progenitor and stem cells. Blood 2001; 97: 3354-60.

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