Development of optimal methods of cryopreservation of human umbilical cord blood hematopoietic cells for transplantation

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Abstract

The purpose of this study was to develop a method for collecting, fractionating cord blood and cryopreservation of hematopoietic stem cells. A method of collecting umbilical cord blood in a built-in Fenwal, Baxter container has been worked out. A method of cord blood fractionation using polyglucine has been developed. The technique of cryopreservation of hematopoietic stem cells using a 5% DMSO solution in combination with polyglucine and freezing in liquid nitrogen vapor at a constant cooling rate has been developed. A computer database has been created and tested to store information about cord blood samples. The developed techniques can be successfully applied for harvesting, long-term storage of hematopoietic cord blood stem cells with subsequent transplantation to patients suffering from oncological diseases.

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About the authors

V. V. Grishina

Bank of Cryopreserved Biomaterials of the N.N. Blokhin Russian Cancer Research Center of the Russian Academy of Medical Sciences

Author for correspondence.
Email: redaktor@celltranspl.ru
Russian Federation, Moscow

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