C-kit-positive pancreas islets cell of rats pancreas as a endocrine cells progenitor during alloxan diabetes
- Authors: Plyushkina AS1, Kaligin MS1, Andreeva DI1, Titova AA1, Valeeva IK.1, Dem'yanov AV1, Gumerova AA1, Kiyasov AP1, Plushkina AS2, Kaligin MS2, Andreeva DI2, Titova AA2, Valeeva IH2, Demyanov AV2, Gumerova AA2,3,1, Kiassov AP2,3,1
-
Affiliations:
- Kazan State Medical University, Kazan
- Kazan (Volga Region) Federal University, Kazan
- Issue: Vol 7, No 3 (2012)
- Pages: 138-141
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121645
- DOI: https://doi.org/10.23868/gc121645
- ID: 121645
Cite item
Open Access
Access granted
Subscription or Fee Access
Abstract
pancreas is the stem cell factor receptor C-kit (CD117) that
plays a main role in differentiation of progenitor endocrine
cells of pancreas islets in prenatal development and persists
after birth. But still the role of C-kit positive cells in islet
-cells regeneration during the diabetes mellitus type I has
not been studied. Thats why the aim of our work was to
study the dynamic of C-kit expression in the pancreas islets
during the experimental alloxan diabetes in rats. The work
was made on 33 rats with the experimental diabetes. Blood
glucose levels, levels of insulin and glucagon were measured.
And also we studied the expression of C-kit, insulin and
glucagon in rat pancreas. The results of the study showed
the C-kit expression after one day of the experimental
hyperglycemia. These cells were also expressed insulin and
glucagon. We suppose that C-kit+-cells, which produce
insulin, were enable to correct disrupted carbohydrate
metabolism during alloxan diabetes.
About the authors
A S Plyushkina
M S Kaligin
D I Andreeva
A A Titova
I Kh Valeeva
A V Dem'yanov
A A Gumerova
A P Kiyasov
A S Plushkina
Kazan State Medical University, KazanKazan State Medical University, Kazan
M S Kaligin
Kazan State Medical University, KazanKazan State Medical University, Kazan
D I Andreeva
Kazan State Medical University, KazanKazan State Medical University, Kazan
A A Titova
Kazan State Medical University, KazanKazan State Medical University, Kazan
I H Valeeva
Kazan State Medical University, KazanKazan State Medical University, Kazan
A V Demyanov
Kazan State Medical University, KazanKazan State Medical University, Kazan
A A Gumerova
Kazan State Medical University, Kazan;Kazan (Volga Region) Federal University, Kazan;Kazan State Medical University, Kazan;Kazan (Volga Region) Federal University, Kazan;
A P Kiassov
Kazan State Medical University, Kazan;Kazan (Volga Region) Federal University, Kazan;Kazan State Medical University, Kazan;Kazan (Volga Region) Federal University, Kazan;
References
- Калигин М.С., Гумерова А.А, Титова М.А. и др. C-kit маркёр стволовых клеток эндокриноцитов поджелудочной железы. Морфо- логия 2011; 140(4): 32-7.
- Закирьянов А.Р., Онищенко Н.А. Возможные пути реализации регенерационной стратегии при лечении сахарного диабета I типа методами клеточной трансплантации. Клеточная трансплантология и тканевая инженерия 2007; 2(II): 23-33.
- Баранов В.Г., Соколоверова И.М., Гаспарян Э.Г. Эксперимен- тальный сахарный диабет. Роль в клинической диабетологии. Л.: Наука; 1983.
- Дедов И.И., Мельниченко Г.А., Фадеев В.Ф. Эндокриноло- гия. М.: ГЭОТАР-Медиа; 2007.
- Орехович В.Н. Современные методы в биохимии. М.: Наука; 1977.
- Herrera P.L., Huarte J., Zufferey R. et al. Ablation of islet endocrine cells by targeted expression of hormone-promoter-driven toxigenes. PNAS USA 1994; 91: 12999-3003.
- Jensen J., Heller R.S., Funder-Nielsen T. et al. Independent development of pancreatic - and -cells from neurogenin3-expressing precursors a role for the notch pathway in repression of premature differentiation. Diabetes 2000; 49: 163-76.
Supplementary files
